[布拉酵母菌减轻2,4,6-三硝基苯磺酸诱导的小鼠结肠炎模型中的肠道炎症：基于基因芯片分析]
[Saccharomyces boulardii reduced intestinal inflammation in mice model of 2,4,6-trinitrobencene sulfonic acid induced colitis: based on microarray].
作者信息
Lee Sang Kil, Kim Hyo Jong, Chi Sung Gil
机构信息
Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
出版信息
Korean J Gastroenterol. 2010 Jan;55(1):33-45. doi: 10.4166/kjg.2010.55.1.33.
BACKGROUND/AIMS: Saccharomyces boulardii has been reported to be beneficial in the treatment of inflammatory bowel disease. The aim of this work was to evaluate the effect of S. boulardii in a mice model of 2,4,6-trinitrobencene sulfonic acid (TNBS) induced colitis and analyze the expression of genes in S. boulardii treated mice by microarray.
METHODS
BALB/c mice received TNBS or TNBS and S. boulardii treatment for 4 days. Microarray was performed on total mRNA form colon, and histologic evaluation was also performed.
RESULTS
In mice treated with S. boulardii, the histological appearance and mortality rate were significantly restored compared with rats receiving only TNBS. Among 330 genes which were altered by both S. boulardii and TNBS (>2 folds), 193 genes were down-regulated by S. boulardii in microarray. Most of genes which were down-regulated by S. bouardii were functionally classified as inflammatory and immune response related genes.
CONCLUSIONS
S. boulardii may reduce colonic inflammation along with regulation of inflammatory and immune responsive genes in TNBS-induced colitis.
背景/目的：据报道，布拉酵母菌对炎症性肠病的治疗有益。本研究旨在评估布拉酵母菌在2,4,6-三硝基苯磺酸（TNBS）诱导的小鼠结肠炎模型中的作用，并通过基因芯片分析经布拉酵母菌处理的小鼠的基因表达情况。
方法
BALB/c小鼠接受TNBS或TNBS与布拉酵母菌联合处理4天。对结肠总mRNA进行基因芯片检测，并进行组织学评估。
结果
与仅接受TNBS处理的小鼠相比，经布拉酵母菌处理的小鼠的组织学表现和死亡率显著恢复。在同时受布拉酵母菌和TNBS影响（变化>2倍）的330个基因中，有193个基因在基因芯片上被布拉酵母菌下调。大多数被布拉酵母菌下调的基因在功能上被归类为与炎症和免疫反应相关的基因。
结论
在TNBS诱导的结肠炎中，布拉酵母菌可能通过调节炎症和免疫反应相关基因来减轻结肠炎症。
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