Kwinta Przemko, Bik-Multanowski Mirosław, Mitkowska Zofia, Tomasik Tomasz, Legutko Magdalena, Pietrzyk Jacek Józef
Department of Pediatrics, Jagiellonian University, Cracow 30-663, Poland.
Pediatr Res. 2008 Dec;64(6):682-8. doi: 10.1203/PDR.0b013e318184edeb.
The aim of the study was to assess the association between bronchopulmonary dysplasia (BPD) and polymorphisms of genes coding for vascular endothelial growth factor (VEGF), transforming growth factor (TGF-[beta]1), insulin-like growth factor (IGF-1), and 5,10-methylenetetrahydrofolate reductase (MTHFR). A sample of 181 newborns with mean gestational age of 28 wk was prospectively evaluated. Molecular analysis of TGF-[beta]1 -800G>A, -509C>T, 10T>C, 25G>C, VEGF -460T>C and 405G>C and MTHFR 677C>T polymorphisms were performed and the number of CA repeats in the promoter region of IGF-1 gene was assessed. The frequency of all TGF-[beta]1, IGF-1, and MTHFR polymorphisms, as well as the frequency of VEGF 405G>C polymorphism was similar in all groups. The newborns with -460TT and -460CT genotypes were significantly overrepresented in the BPD groups compared with the no BPD group. Multivariate analysis revealed that carrying T allele increased the risk of BPD by 9% (95%CI: 2-14%) above the baseline risk established for given gestational age, length of oxygen therapy, and sex. Based on our data from a single center, we propose that VEGF -460T>C polymorphism may influence the risk of BPD.
本研究旨在评估支气管肺发育不良（BPD）与血管内皮生长因子（VEGF）、转化生长因子（TGF-β1）、胰岛素样生长因子（IGF-1）及5,10-亚甲基四氢叶酸还原酶（MTHFR）编码基因多态性之间的关联。对181例平均胎龄为28周的新生儿样本进行了前瞻性评估。进行了TGF-β1 -800G>A、-509C>T、10T>C、25G>C、VEGF -460T>C和405G>C以及MTHFR 677C>T多态性的分子分析，并评估了IGF-1基因启动子区域CA重复序列的数量。所有组中所有TGF-β1、IGF-1和MTHFR多态性的频率以及VEGF 405G>C多态性的频率均相似。与无BPD组相比，BPD组中-460TT和-460CT基因型的新生儿明显过多。多变量分析显示，携带T等位基因使BPD风险比给定胎龄、氧疗时长和性别所确定的基线风险增加9%（95%CI：2-14%）。基于我们单中心的数据，我们提出VEGF -460T>C多态性可能影响BPD风险。
