Ma Hui-Ping, Jia Zheng-Ping, Cheng Ke-Ming, Zhang Ru-Xue, Li Mao-Xing, Wang Juan
Department of Pharmacy, Lanzhou General Hospital, PLA, Lanzhou 730050, China.
Zhongguo Zhong Yao Za Zhi. 2008 Apr;33(8):928-31.
To investigate the effects of total flavonoid extract of Epimedium sagittatum (TFE) on the proliferation and differentiation of newborn rat calvarial osteoblasts (ROB).
TFE was supplemented into the culture medium of ROB at 0. 1, 1, 10 and 100 microg x mL(-1) respectively. The serum of rats administered TFES (SRAT) was also added into the medium in a parallel treatment at 2.5%, 5% and 10% respectively. Their effects on cell proliferation and differentiation was studied by MTT and the analysis of osteogenic differentiation marks.
TFE had no appreciable and on cell proliferation and differentiation at any concentration. However, 2.5% and 5% SRAT stimulated cell proliferation strongly and, 5% SRAT significantly promoted the maturation and function of osteoblast by improving the alkaline phosphatase activity, osteocalcin secretion, calcium deposition and the number of mineralized nodular structures.
The metabolites of TFE should be the anti-osteoporosis constitutes of Epimedium sagittatum.
研究箭叶淫羊藿总黄酮提取物（TFE）对新生大鼠颅骨成骨细胞（ROB）增殖和分化的影响。
分别以0.1、1、10和100μg·mL⁻¹的浓度将TFE添加到ROB的培养基中。同时，分别以2.5%、5%和10%的比例将给予TFE的大鼠血清（SRAT）平行添加到培养基中。通过MTT法和成骨分化标志物分析研究它们对细胞增殖和分化的影响。
TFE在任何浓度下对细胞增殖和分化均无明显影响。然而，2.5%和5%的SRAT强烈刺激细胞增殖，5%的SRAT通过提高碱性磷酸酶活性、骨钙素分泌、钙沉积和矿化结节结构数量，显著促进成骨细胞的成熟和功能。
TFE的代谢产物应为箭叶淫羊藿抗骨质疏松的成分。
