Card G P, Vezza A C, Bishop D H, Compans R W
Department of Microbiology, The Medical Center, University of Alabama in Birmingham, Birmingham, Alabama 35294, USA.
Virology. 1977 Nov;83(1):84-95. doi: 10.1016/0042-6822(77)90212-4.
Tacaribe and Tamiami viruses were grown in BHK-21 monolayers and purified by centrifugation in combination glycerol-tartrate gradients followed by sucrose gradient centrifugation. The major polypeptides of Tacaribe and Tamiami virions were shown to differ from those of Pichinde virions when suitably labeled preparations of the three viruses were coelectrophoresed in SDS-polyacrylamide gels. Tacaribe and Tamiami virions each contained two major polypeptides, a nonglycosylated protein of molecular weight 68,000 and 66,000, respectively, and a single glycoprotein size-class of molecular weight 42,000 and 44,000, respectively. The nonglycosylated protein is associated with the viral RNA in a nucleoprotein structure which can be isolated by equilibrium centrifugation in CsCl. The glycoproteins were selectively removed from virions by chymotrypsin treatment, which produces essentially spikeless particles. Tacaribe and Tamiami virions also contain a minor polypeptide species of molecular weight 79,000 and 77,000, respectively.
塔卡里比病毒和塔米亚米病毒在BHK - 21单层细胞中培养，先通过在甘油 - 酒石酸盐梯度中离心，然后进行蔗糖梯度离心来纯化。当三种病毒的适当标记制剂在SDS - 聚丙烯酰胺凝胶中进行共电泳时，塔卡里比病毒和塔米亚米病毒粒子的主要多肽显示出与皮钦德病毒粒子的不同。塔卡里比病毒和塔米亚米病毒粒子各自含有两种主要多肽，分别是分子量为68,000和66,000的非糖基化蛋白，以及分子量分别为42,000和44,000的单一糖蛋白大小类别。非糖基化蛋白在核蛋白结构中与病毒RNA相关联，该结构可通过在CsCl中进行平衡离心分离出来。通过胰凝乳蛋白酶处理可从病毒粒子中选择性去除糖蛋白，这会产生基本上无刺的颗粒。塔卡里比病毒和塔米亚米病毒粒子还分别含有分子量为79,000和77,000的次要多肽种类。
