Ngan Dai Hue, Hoai Ho Thi Cam, Huong Le Mai, Hansen Poul Erik, Vang Ole
Department of Chemistry, College of Natural Sciences, National University of Ho Chi Minh City, 227 Nguyen Van Cu St., Dist. 5, Ho Chi Minh City, Vietnam.
Nat Prod Res. 2008;22(11):942-9. doi: 10.1080/14786410701647119.
Five crude extracts were made from leaves and stems of Jasminum subtriplinerve Blume (Oleaceae) and investigated for antimicrobial, antioxidant and cytotoxic activities. The extractions were done with petroleum ether, ethyl acetate, ethanol, methanol or water. All extracts exhibited anti-bacterial activity except the water fraction. On the other hand, all extracts exhibit antioxidant activity except the petroleum ether fraction using the DPPH radical scavenging assay. Only the petroleum ether fraction showed a cytotoxicity activity against tested cell-lines, Hep-G2 and RD with IC(50) values of 19.2 and 20 microg mL(-1), respectively. From the petroleum ether and ethyl acetate extracts, two triterpenes namely 3beta-acetyl-oleanolic acid and lup-20-en-3beta-ol and a sterol, stigmast-5-en-3beta-ol were isolated. The structure of those compounds were elucidated by spectrometric methods IR, MS, 1D-NMR, 2D-NMR and simulated ACD/NMR spectra. The data presented here indicate that J. subtriplinerve do contain compounds with interesting biological activity.
从素馨（木犀科）的叶和茎中制备了五种粗提物，并对其抗菌、抗氧化和细胞毒性活性进行了研究。提取分别使用石油醚、乙酸乙酯、乙醇、甲醇或水进行。除水相提取物外，所有提取物均表现出抗菌活性。另一方面，使用DPPH自由基清除试验，除石油醚提取物外，所有提取物均表现出抗氧化活性。只有石油醚提取物对受试细胞系Hep-G2和RD显示出细胞毒性活性，IC50值分别为19.2和20μg mL-1。从石油醚和乙酸乙酯提取物中分离出两种三萜，即3β-乙酰齐墩果酸和羽扇豆-20-烯-3β-醇，以及一种甾醇，豆甾-5-烯-3β-醇。通过光谱方法IR、MS、1D-NMR、2D-NMR和模拟ACD/NMR光谱对这些化合物的结构进行了阐明。此处提供的数据表明，素馨确实含有具有有趣生物活性的化合物。
