Lin Jing-Wen, Chen Qian-Ming, Li Sheng-Fu, Song Hui-Yun, Long Dan, Zhou Hong-Mei
Department of Oral Medicine, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Mar;39(2):184-7.
The aim of this study was in vitro to investigate the effects of oral carcinoma-associated fibroblasts (CAFs) on the proliferation of lingual carcinoma cells.
The interaction model between primary oral CAFs and a lingual carcinoma cell line Tca8113 was established for this study project. The effects of CAFs on the viability and cell cycle of Tca8113 were investigated through morphological observation, MTT assay and flow cytometry.
After oral CAFs interacted with Tca8113 directly, Tca8113 showed the cellular shape changes in morphology; compared with normal fibroblasts (NFs), CAFs enhanced the viability of Tca8113 (P<0.05), and increased the percentage of carcinoma cells in S phase and G2 phase (48.1% vs 40.0%).
Oral CAFs can promote the proliferation of the lingual carcinoma cell line Tca8113 in vitro, and may play a key role in the progression of oral squamous cell carcinoma (OSCC).
本研究旨在体外探究口腔癌相关成纤维细胞（CAFs）对舌癌细胞增殖的影响。
本研究项目建立了原代口腔CAFs与舌癌细胞系Tca8113之间的相互作用模型。通过形态学观察、MTT法和流式细胞术研究CAFs对Tca8113细胞活力和细胞周期的影响。
口腔CAFs与Tca8113直接相互作用后，Tca8113在形态上呈现细胞形状改变；与正常成纤维细胞（NFs）相比，CAFs增强了Tca8113的细胞活力（P<0.05），并增加了处于S期和G2期的癌细胞百分比（48.1%对40.0%）。
口腔CAFs可在体外促进舌癌细胞系Tca8113的增殖，可能在口腔鳞状细胞癌（OSCC）进展中起关键作用。
