Lin Jingwen, Liu Chuanxia, Ge Lin, Gao Qinghong, He Xin, Liu Ying, Li Shengfu, Zhou Min, Chen Qianming, Zhou Hongmei
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, Sichuan, China.
Tumour Biol. 2011 Jun;32(3):597-602. doi: 10.1007/s13277-011-0158-5. Epub 2011 Feb 22.
Carcinoma-associated fibroblasts (CAFs) have been confirmed to play an important role in the occurrence and development of many kinds of tumors. Regarding proliferation as one manifestation of malignance, the objective was to observe the effects of oral CAFs on the proliferation of oral squamous carcinoma cells (OSCC) and to explore the role of keratinocyte growth factor (KGF) in this process. The results showed that oral CAFs secreted a higher level of KGF than oral normal fibroblasts (NFs), and the conditioned medium of CAFs could increase the viability of carcinoma cells and promote more of them into G2 and S phase. However, after blocking with KGF antibody, the viability and cell cycle of Tca8113 cultured with CAFs conditioned medium changed to be similar with NFs control groups. It was concluded that CAFs could promote the proliferation of OSCC through secreting high levels of KGF. These findings support the use of carcinoma-associated fibroblasts as a novel target in anticancer therapy.
癌相关成纤维细胞（CAFs）已被证实，在多种肿瘤的发生和发展中发挥重要作用。鉴于增殖是恶性肿瘤的一种表现形式，本研究旨在观察口腔CAFs对口腔鳞状癌细胞（OSCC）增殖的影响，并探讨角质形成细胞生长因子（KGF）在此过程中的作用。结果显示，口腔CAFs分泌的KGF水平高于口腔正常成纤维细胞（NFs），且CAFs的条件培养基可提高癌细胞的活力，并促使更多癌细胞进入G2期和S期。然而，在用KGF抗体阻断后，用CAFs条件培养基培养的Tca8113细胞的活力和细胞周期变化，与NFs对照组相似。研究得出结论，CAFs可通过分泌高水平的KGF促进OSCC的增殖。这些发现支持将癌相关成纤维细胞作为抗癌治疗的新靶点。
