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结晶豇豆花叶病毒

Crystalline cowpea mosaic virus.

作者信息

White J M, Johnson J E

机构信息

Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.

出版信息

Virology. 1980 Feb;101(1):319-24. doi: 10.1016/0042-6822(80)90513-9.

DOI:10.1016/0042-6822(80)90513-9
PMID:18631640
Abstract

Crystals of Cowpea mosaic virus displaying a high degree of order have been grown in two different forms. Hexagonal crystals, space group P6(1)22 or P6(5)22 with a = 450 A and c = 1038 A containing six virus particles per cell, have been grown in sodium citrate buffer at pH 4.9. Each particle has four nearest neighbors at 284 A. Cubic crystals, with space group I23 and a = 308 A , containing two particles per cell were grown from ammonium sulfate at pH 4.9. In the cubic crystals, the tetrahedral subgroup of the icosahedral particle symmetry is expressed as part of the crystal lattice, with each particle occupying tetrahedral crystallographic positions. Contacts of 268 A occur between particles in contact along threefold icosahedral symmetry axes.

摘要

豇豆花叶病毒已形成两种高度有序的晶体形式。在pH值为4.9的柠檬酸钠缓冲液中生长出了六方晶体,其空间群为P6(1)22或P6(5)22,a = 450 Å,c = 1038 Å,每个晶胞包含六个病毒粒子。每个粒子在284 Å处有四个最近邻粒子。立方晶体的空间群为I23,a = 308 Å,每个晶胞包含两个粒子,是在pH值为4.9的硫酸铵中生长得到的。在立方晶体中,二十面体粒子对称性的四面体子群表现为晶格的一部分,每个粒子占据四面体晶体学位置。沿二十面体三重对称轴接触的粒子之间的间距为268 Å。

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引用本文的文献

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Confessions of an icosahedral virus crystallographer.一位二十面体病毒晶体学家的自白。
Microscopy (Oxf). 2013 Feb;62(1):69-79. doi: 10.1093/jmicro/dfs097. Epub 2013 Jan 4.
2
Magnification calibration and the determination of spherical virus diameters using cryo-microscopy.使用冷冻显微镜进行放大倍率校准和球形病毒直径的测定。
Ultramicroscopy. 1989 Jul-Aug;30(3):281-97. doi: 10.1016/0304-3991(89)90057-0.