Dehennaut Vanessa, Lefebvre Tony, Leroy Yves, Vilain Jean-Pierre, Michalski Jean-Claude, Bodart Jean-François
UMR-CNRS 8576, Unité de Glycobiologie Structurale et Fonctionnelle, IFR 147, USTL, 59655, Villeneuve d'Ascq, France.
Glycoconj J. 2009 Apr;26(3):301-11. doi: 10.1007/s10719-008-9166-0. Epub 2008 Jul 17.
Little is known about the impact of O-linked-N-acetylglucosaminylation (O-GlcNAc) in gametes production and developmental processes. Here we investigated changes in O-GlcNAc, UDP-GlcNAc and O-GlcNAc transferase (OGT) levels in Xenopus laevis from oogenesis to embryo hatching. We showed that in comparison to stage VI, stages I-V oocytes expressed higher levels of O-GlcNAc correlating changes in OGT expression, but not in UDP-GlcNAc pools. Upon progesterone stimulation, an O-GlcNAc level burst occurred during meiotic resumption long before MPF and Mos-Erk2 pathways activations. Finally, we observed high levels of O-GlcNAc, UDP-GlcNAc and OGT during segmentation that decreased concomitantly at the onset of gastrulation. Nevertheless, no correlation between the glycosylation, the nucleotide-sugar and the glycosyltransferase was observed after neurulation. Our results show that O-GlcNAc is regulated throughout oogenesis and development within a complex pattern and suggest that dysfunctions in the dynamics of this glycosylation could lead to developmental abnormalities.
关于O-连接的N-乙酰葡糖胺化（O-GlcNAc）在配子产生和发育过程中的影响，人们了解甚少。在此，我们研究了非洲爪蟾从卵子发生到胚胎孵化过程中O-GlcNAc、UDP-GlcNAc和O-GlcNAc转移酶（OGT）水平的变化。我们发现，与VI期相比，I-V期卵母细胞中O-GlcNAc的表达水平更高，这与OGT表达的变化相关，但与UDP-GlcNAc池的变化无关。在孕酮刺激下，在减数分裂恢复期间，早在MPF和Mos-Erk2途径激活之前，O-GlcNAc水平就会出现爆发。最后，我们观察到在卵裂期间O-GlcNAc、UDP-GlcNAc和OGT水平较高，而在原肠胚形成开始时这些水平会随之下降。然而，在神经胚形成后，未观察到糖基化、核苷酸糖和糖基转移酶之间的相关性。我们的结果表明，O-GlcNAc在整个卵子发生和发育过程中以复杂的模式受到调控，并表明这种糖基化动态过程中的功能障碍可能导致发育异常。
