Sun Xiao-Yun, Shi Jinxia, Han Lei, Su Yin, Li Zhan-Guo
Department of Rheumatology and Immunology, People's Hospital, Peking University Medical School, Beijing, China.
J Clin Lab Anal. 2008;22(4):271-7. doi: 10.1002/jcla.20248.
To investigate the specificity, sensitivity, and concomitant presence of antibodies against histones (H), histone H1 (H1), and histone H3 (H3) in patients with systemic lupus erythematosus (SLE) and analyze their association with SLE. Serum IgG anti-histones antibodies were detected by enzyme-linked immunosorbent assay in 144 SLE patients consisting of 24 neuropsychiatric lupus (NPSLE), 65 lupus nephritis (LN), and 55 SLE, 100 other rheumatic diseases patients, as well as 40 healthy controls. Clinical and biological parameters of the patients were also evaluated. Anti-H, anti-H1, and anti-H3 antibodies yielded a sensitivity of approximately 33% and a specificity of more than 93% for SLE, which was comparable to that found for anti-double-stranded DNA (anti-dsDNa) antibodies. More significantly, anti-histone antibody is found in approximately 50% of patients with NPSLE compared with LN. Moreover, the titers of anti-histones antibodies of NPSLE patients were significantly higher than that of patients with SLE and LN. The sequential analysis revealed a close correlation of anti-H and anti-H1 antibodies with SLE disease activity. There was an approximate 30% positive rate of anti-histones antibodies in 144 SLE patients lacking anti-nucleosome, anti-mDNA, anti-Sm, and anti-dsDNA antibodies. Antibodies to histones H1 and H3 are markers with high specificity of 93.6-96.4% for SLE. The anti-histone antibody markers are prevalent in approximately 50% of NPSLE. Furthermore, there was a strong correlation with SLE disease activity index and levels of antibodies to histones and H1.
为研究系统性红斑狼疮（SLE）患者中抗组蛋白（H）、组蛋白H1（H1）和组蛋白H3（H3）抗体的特异性、敏感性及共存情况，并分析它们与SLE的相关性。采用酶联免疫吸附测定法检测了144例SLE患者（包括24例神经精神性狼疮（NPSLE）、65例狼疮性肾炎（LN）和55例SLE患者）、100例其他风湿性疾病患者以及40例健康对照者血清中的IgG抗组蛋白抗体。还评估了患者的临床和生物学参数。抗H、抗H1和抗H3抗体对SLE的敏感性约为33%，特异性超过93%，这与抗双链DNA（抗dsDNA）抗体的情况相当。更显著的是，与LN患者相比，约50%的NPSLE患者中发现了抗组蛋白抗体。此外，NPSLE患者的抗组蛋白抗体滴度显著高于SLE和LN患者。序贯分析显示抗H和抗H1抗体与SLE疾病活动密切相关。在144例缺乏抗核小体、抗线粒体DNA、抗Sm和抗dsDNA抗体的SLE患者中，抗组蛋白抗体的阳性率约为30%。H1和H3组蛋白抗体是SLE特异性高达93.6 - 96.4%的标志物。抗组蛋白抗体标志物在约50%的NPSLE中普遍存在。此外，SLE疾病活动指数与组蛋白和H1抗体水平之间存在很强的相关性。
