Lu Yuanyuan, Luo Jianguang, Xu Deran, Huang Xuefeng, Kong Lingyi
Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, China.
Rapid Commun Mass Spectrom. 2008 Aug;22(16):2447-52. doi: 10.1002/rcm.3630.
High-performance liquid chromatography with an evaporative light scattering detector and electrospray ionization multistage tandem mass spectrometry (HPLC/ELSD/ESI-MS(n)) was used to identify spirostanol saponins in a saponin extract of Solanum torvum. The fragmentation behavior of saponins was studied using ESI-MS(1-3) in positive ion mode. The MS(n) spectra of the M+H ions provide structural information including aglycone type and the nature and sequence of sugars. The use of ELSD allowed the profiling of the nonchromophore-containing saponins in this plant. The MS analysis established in this study with known saponins was successfully applied to tentatively identify two new siprostanol glycosides, neosolaspigenin 6-O-beta-D-quinovopyranoside and solagenin 6-O-[beta-D-xylopyranosyl-(1 --> 3)-O-beta-D-quinovopyranoside].
采用带蒸发光散射检测器的高效液相色谱法和电喷雾电离多级串联质谱法（HPLC/ELSD/ESI-MS(n)）对刺茄皂苷提取物中的螺旋甾烷醇皂苷进行鉴定。在正离子模式下，使用ESI-MS(1-3)研究了皂苷的裂解行为。M+H离子的MS(n)光谱提供了包括苷元类型以及糖的性质和序列在内的结构信息。蒸发光散射检测器的使用使得对该植物中不含发色团的皂苷进行了分析。本研究中利用已知皂苷建立的质谱分析方法成功地用于初步鉴定两种新的螺旋甾烷醇糖苷，即新刺茄皂苷元6-O-β-D-奎诺吡喃糖苷和茄皂苷元6-O-[β-D-木糖吡喃糖基-(1→3)-O-β-D-奎诺吡喃糖苷]。
