天然鞘氨醇单胞菌糖脂激活自然杀伤T细胞的能力差异很大。
Natural Sphingomonas glycolipids vary greatly in their ability to activate natural killer T cells.
作者信息
Kinjo Yuki, Pei Bo, Bufali Simone, Raju Ravinder, Richardson Stewart K, Imamura Masakazu, Fujio Masakazu, Wu Douglass, Khurana Archana, Kawahara Kazuyoshi, Wong Chi-Huey, Howell Amy R, Seeberger Peter H, Kronenberg Mitchell
机构信息
Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA.
出版信息
Chem Biol. 2008 Jul 21;15(7):654-64. doi: 10.1016/j.chembiol.2008.05.012.
Mouse natural killer T (NKT) cells expressing an invariant T cell antigen receptor (TCR) recognize glycosphingolipids (GSLs) from Sphingomonas bacteria. The synthetic antigens previously tested, however, were designed to closely resemble the potent synthetic agonist alpha-galactosyl ceramide (alphaGalCer), which contains a monosaccharide and a C18:0 sphingosine lipid. Some Sphingomonas bacteria, however, also have oligosaccharide-containing GSLs, and they normally synthesize several GSLs with different sphingosine chains including one with a cyclopropyl ring-containing C21:0 (C21cycl) sphingosine. Here we studied the stimulation of NKT cells with synthetic GSL antigens containing natural tetrasaccharide sugars, or the C21cycl sphingosine. Our results indicate that there is a great degree of variability in the antigenic potency of different natural Sphingomonas glycolipids, with the C21cycl sphingosine having intermediate potency and the oligosaccharide-containing antigens exhibiting limited or no stimulatory capacity.
表达恒定T细胞抗原受体（TCR）的小鼠自然杀伤T（NKT）细胞可识别来自鞘氨醇单胞菌属细菌的糖鞘脂（GSL）。然而，之前测试的合成抗原旨在与强效合成激动剂α-半乳糖基神经酰胺（αGalCer）高度相似，αGalCer含有一个单糖和一条C18:0鞘氨醇脂质链。然而，一些鞘氨醇单胞菌属细菌也含有含寡糖的GSL，并且它们通常会合成几种具有不同鞘氨醇链的GSL，其中一种含有含环丙基的C21:0（C21cycl）鞘氨醇。在此，我们研究了含有天然四糖或C21cycl鞘氨醇的合成GSL抗原对NKT细胞的刺激作用。我们的结果表明，不同天然鞘氨醇单胞菌糖脂的抗原效力存在很大差异，C21cycl鞘氨醇具有中等效力，而含寡糖的抗原表现出有限的或无刺激能力。
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