跨膜亚基 K 中的 NADH：泛醌氧化还原酶的保守负电荷决定了其对 YidC 进行膜插入的依赖性。
Conserved negative charges in the transmembrane segments of subunit K of the NADH:ubiquinone oxidoreductase determine its dependence on YidC for membrane insertion.
机构信息
From the Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, the Zernike Institute for Advanced Materials, and the Kluyver Center for Genomics of Industrial Fermentation, University of Groningen, Kerklaan 30, NL-9751 NN Haren, The Netherlands.
From the Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, the Zernike Institute for Advanced Materials, and the Kluyver Center for Genomics of Industrial Fermentation, University of Groningen, Kerklaan 30, NL-9751 NN Haren, The Netherlands.
出版信息
J Biol Chem. 2010 Feb 5;285(6):3575-3581. doi: 10.1074/jbc.M109.051128. Epub 2009 Dec 3.
Abstract
All members of the Oxa1/Alb3/YidC family have been implicated in the biogenesis of respiratory and energy transducing proteins. In Escherichia coli, YidC functions together with and independently of the Sec system. Although the range of proteins shown to be dependent on YidC continues to increase, the exact role of YidC in insertion remains enigmatic. Here we show that YidC is essential for the insertion of subunit K of the NADH:ubiquinone oxidoreductase and that the dependence is due to the presence of two conserved glutamate residues in the transmembrane segments of subunit K. The results suggest a model in which YidC serves as a membrane chaperone for the insertion of the less hydrophobic, negatively charged transmembrane segments of NuoK.
摘要
Oxa1/Alb3/YidC 家族的所有成员都与呼吸和能量转导蛋白的生物发生有关。在大肠杆菌中，YidC 与 Sec 系统一起发挥作用，也可以独立发挥作用。尽管越来越多的蛋白质被证明依赖于 YidC，但 YidC 在插入过程中的确切作用仍然是个谜。在这里，我们表明 YidC 对于 NADH：泛醌氧化还原酶亚基 K 的插入是必不可少的，并且这种依赖性是由于亚基 K 的跨膜片段中存在两个保守的谷氨酸残基。结果表明，YidC 作为一种膜伴侣，用于插入 NuoK 的疏水性较小、带负电荷的跨膜片段。
相似文献
1
Conserved negative charges in the transmembrane segments of subunit K of the NADH:ubiquinone oxidoreductase determine its dependence on YidC for membrane insertion.跨膜亚基 K 中的 NADH：泛醌氧化还原酶的保守负电荷决定了其对 YidC 进行膜插入的依赖性。
J Biol Chem. 2010 Feb 5;285(6):3575-3581. doi: 10.1074/jbc.M109.051128. Epub 2009 Dec 3.
2
Molecular communication of the membrane insertase YidC with translocase SecYEG affects client proteins.膜插入酶 YidC 与易位子 SecYEG 的分子通讯影响靶蛋白。
Sci Rep. 2021 Feb 16;11(1):3940. doi: 10.1038/s41598-021-83224-x.
3
Polarity and charge of the periplasmic loop determine the YidC and sec translocase requirement for the M13 procoat lep protein.周质环的极性和电荷决定了M13原衣壳lep蛋白对YidC和Sec转位酶的需求。
J Biol Chem. 2014 Jan 10;289(2):1023-32. doi: 10.1074/jbc.M113.522250. Epub 2013 Nov 25.
4
YidC is involved in the biogenesis of anaerobic respiratory complexes in the inner membrane of Escherichia coli.YidC参与大肠杆菌内膜中厌氧呼吸复合物的生物合成。
J Biol Chem. 2008 Oct 3;283(40):26921-7. doi: 10.1074/jbc.M804490200. Epub 2008 Jul 17.
5
Role of the Cytosolic Loop C2 and the C Terminus of YidC in Ribosome Binding and Insertion Activity.YidC的胞质环C2和C末端在核糖体结合及插入活性中的作用
J Biol Chem. 2015 Jul 10;290(28):17250-61. doi: 10.1074/jbc.M115.650309. Epub 2015 May 28.
6
The role of the strictly conserved positively charged residue differs among the Gram-positive, Gram-negative, and chloroplast YidC homologs.在革兰氏阳性菌、革兰氏阴性菌以及叶绿体的YidC同源物中，严格保守的带正电荷残基的作用有所不同。
J Biol Chem. 2014 Dec 19;289(51):35656-67. doi: 10.1074/jbc.M114.595082. Epub 2014 Oct 30.
7
The Sec-independent function of Escherichia coli YidC is evolutionary-conserved and essential.大肠杆菌YidC的Sec非依赖性功能在进化上保守且至关重要。
J Biol Chem. 2005 Apr 1;280(13):12996-3003. doi: 10.1074/jbc.M414094200. Epub 2005 Jan 25.
8
Membrane biogenesis of subunit II of cytochrome bo oxidase: contrasting requirements for insertion of N-terminal and C-terminal domains.细胞色素 bo 氧化酶亚基 II 的膜生物合成：N 端和 C 端结构域插入的不同要求。
J Mol Biol. 2006 Apr 14;357(5):1428-36. doi: 10.1016/j.jmb.2006.01.030. Epub 2006 Jan 26.
9
New Insights into Amino-Terminal Translocation as Revealed by the Use of YidC and Sec Depletion Strains.使用 YidC 和 Sec 耗竭菌株揭示的氨端易位的新见解。
J Mol Biol. 2019 Mar 1;431(5):1025-1037. doi: 10.1016/j.jmb.2019.01.006. Epub 2019 Jan 9.
10
Defining the regions of Escherichia coli YidC that contribute to activity.确定大肠杆菌YidC中对活性有贡献的区域。
J Biol Chem. 2003 Dec 5;278(49):48965-72. doi: 10.1074/jbc.M307362200. Epub 2003 Sep 22.
引用本文的文献
1
A unifying model for membrane protein biogenesis.膜蛋白生物发生的统一模型。
Nat Struct Mol Biol. 2024 Jul;31(7):1009-1017. doi: 10.1038/s41594-024-01296-5. Epub 2024 May 29.
2
Cotranslational Biogenesis of Membrane Proteins in Bacteria.细菌中膜蛋白的共翻译生物合成
Front Mol Biosci. 2022 Apr 29;9:871121. doi: 10.3389/fmolb.2022.871121. eCollection 2022.
3
Fates of Sec, Tat, and YidC Translocases in Mitochondria and Other Eukaryotic Compartments.Sec、Tat 和 YidC 易位子在线粒体和其他真核生物隔室中的命运。
Mol Biol Evol. 2021 Dec 9;38(12):5241-5254. doi: 10.1093/molbev/msab253.
4
Lateral gate dynamics of the bacterial translocon during cotranslational membrane protein insertion.细菌易位通道在共翻译膜蛋白插入过程中的横向门控动力学。
Proc Natl Acad Sci U S A. 2021 Jun 29;118(26). doi: 10.1073/pnas.2100474118.
5
A Celecoxib Derivative Eradicates Antibiotic-Resistant and Biofilms by Targeting YidC2 Translocase.一种塞来昔布衍生物通过靶向 YidC2 移位酶根除抗生素耐药性和生物膜。
Int J Mol Sci. 2020 Dec 7;21(23):9312. doi: 10.3390/ijms21239312.
6
Sec translocon has an insertase-like function in addition to polypeptide conduction through the channel.信号肽转运通道除了能引导多肽通过通道外，还具有类似插入酶的功能。
F1000Res. 2019 Dec 20;8. doi: 10.12688/f1000research.21065.1. eCollection 2019.
7
The WRB Subunit of the Get3 Receptor is Required for the Correct Integration of its Partner CAML into the ER.Get3 受体的 WRB 亚基对于其伴侣 CAML 正确整合到内质网中是必需的。
Sci Rep. 2019 Aug 15;9(1):11887. doi: 10.1038/s41598-019-48363-2.
8
A Bacterial Stress Response Regulates Respiratory Protein Complexes To Control Envelope Stress Adaptation.细菌应激反应调节呼吸蛋白复合物以控制包膜应激适应。
J Bacteriol. 2017 Sep 19;199(20). doi: 10.1128/JB.00153-17. Print 2017 Oct 15.
9
Role of the Cytosolic Loop C2 and the C Terminus of YidC in Ribosome Binding and Insertion Activity.YidC的胞质环C2和C末端在核糖体结合及插入活性中的作用
J Biol Chem. 2015 Jul 10;290(28):17250-61. doi: 10.1074/jbc.M115.650309. Epub 2015 May 28.
10
YidC/Alb3/Oxa1 Family of Insertases.插入酶的YidC/Alb3/Oxa1家族。
J Biol Chem. 2015 Jun 12;290(24):14866-74. doi: 10.1074/jbc.R115.638171. Epub 2015 May 6.
本文引用的文献
1
Bacillus subtilis SpoIIIJ and YqjG function in membrane protein biogenesis.枯草芽孢杆菌SpoIIIJ和YqjG在膜蛋白生物合成中发挥作用。
J Bacteriol. 2009 Nov;191(21):6749-57. doi: 10.1128/JB.00853-09. Epub 2009 Aug 28.
2
The conserved third transmembrane segment of YidC contacts nascent Escherichia coli inner membrane proteins.YidC保守的第三个跨膜片段与新生的大肠杆菌内膜蛋白接触。
J Biol Chem. 2008 Dec 12;283(50):34635-42. doi: 10.1074/jbc.M804344200. Epub 2008 Oct 6.
3
YidC is involved in the biogenesis of anaerobic respiratory complexes in the inner membrane of Escherichia coli.YidC参与大肠杆菌内膜中厌氧呼吸复合物的生物合成。
J Biol Chem. 2008 Oct 3;283(40):26921-7. doi: 10.1074/jbc.M804490200. Epub 2008 Jul 17.
4
Assembly of the Escherichia coli NADH:ubiquinone oxidoreductase (complex I).大肠杆菌NADH：泛醌氧化还原酶（复合体I）的组装。
Biochim Biophys Acta. 2008 Jul-Aug;1777(7-8):735-9. doi: 10.1016/j.bbabio.2008.03.003. Epub 2008 Mar 15.
5
Mechanism and hydrophobic forces driving membrane protein insertion of subunit II of cytochrome bo 3 oxidase.细胞色素bo 3氧化酶亚基II膜蛋白插入的机制及疏水作用力
J Mol Biol. 2008 Feb 1;375(5):1282-92. doi: 10.1016/j.jmb.2007.11.054. Epub 2007 Nov 22.
6
Knockdown of human Oxa1l impairs the biogenesis of F1Fo-ATP synthase and NADH:ubiquinone oxidoreductase.抑制人类Oxa1l会损害F1Fo - ATP合酶和NADH：泛醌氧化还原酶的生物合成。
J Mol Biol. 2007 Nov 23;374(2):506-16. doi: 10.1016/j.jmb.2007.09.044. Epub 2007 Sep 20.
7
Oxa1 directly interacts with Atp9 and mediates its assembly into the mitochondrial F1Fo-ATP synthase complex.Oxa1直接与Atp9相互作用，并介导其组装到线粒体F1Fo-ATP合酶复合体中。
Mol Biol Cell. 2007 May;18(5):1897-908. doi: 10.1091/mbc.e06-10-0925. Epub 2007 Mar 7.
8
The mechanosensitive channel protein MscL is targeted by the SRP to the novel YidC membrane insertion pathway of Escherichia coli.机械敏感通道蛋白MscL被信号识别颗粒靶向至大肠杆菌新的YidC膜插入途径。
J Mol Biol. 2007 Jan 26;365(4):995-1004. doi: 10.1016/j.jmb.2006.10.083. Epub 2006 Oct 28.
9
YidC-mediated membrane insertion of assembly mutants of subunit c of the F1F0 ATPase.YidC介导的F1F0 ATP合酶c亚基组装突变体的膜插入。
J Biol Chem. 2006 Oct 6;281(40):29762-8. doi: 10.1074/jbc.M605317200. Epub 2006 Jul 31.
10
Subunit a of cytochrome o oxidase requires both YidC and SecYEG for membrane insertion.细胞色素 o 氧化酶的亚基 a 插入膜中既需要 YidC 也需要 SecYEG。
J Biol Chem. 2006 May 5;281(18):12248-52. doi: 10.1074/jbc.M600048200. Epub 2006 Mar 2.
Zotero 插件