Wilton J M, Renggli H H, Lehner T
Immunology. 1977 Jun;32(6):955-61.
Polymorphonuclear leucocytes from the gingival crevice (CREV-PMN) in man have a defective capacity to phagocytose Candida albicans blastospores. Phagocytosis of zymosan particles, which detect C3b receptors, is also impaired but ingestion of latex beads coated with heat-aggregated IgG, which detects Fc receptors, is normal compared to peripheral blood polymorphonuclear leucocytes (PB-PMN). If phagocytosis is inhibited by Cytochalasin B, fewer CREV-PMN bind Candida and zymosan but the binding of IgG-coated latex beads remains unchanged. CREV-PMN have IgG (88%), IgM (45%) and C3 (48%) on their cell membrane, whilst less than 5% of PB-PMN have any of these components. Incubation of PB-PMN in fluid from the gingival crevice confers surface IgG and C3 to the cells. Such treatment also inhibits the subsequent binding of IgG coated latex beads. The results suggest that the deficiency of phagocytosis by CREV-PMN is due to decreased binding of particles to the C3b receptor of PMN, whilst the Fc receptor system remains intact.
人类牙龈沟中的多形核白细胞（CREV-PMN）吞噬白色念珠菌芽生孢子的能力存在缺陷。检测C3b受体的酵母聚糖颗粒的吞噬作用也受到损害，但与外周血多形核白细胞（PB-PMN）相比，检测Fc受体的热聚集IgG包被的乳胶珠的摄取是正常的。如果用细胞松弛素B抑制吞噬作用，结合念珠菌和酵母聚糖的CREV-PMN会减少，但IgG包被乳胶珠的结合保持不变。CREV-PMN的细胞膜上有IgG（88%）、IgM（45%）和C3（48%），而PB-PMN中只有不到5%的细胞有这些成分中的任何一种。将PB-PMN置于牙龈沟液中孵育会使细胞表面出现IgG和C3。这种处理也会抑制随后IgG包被乳胶珠与之结合。结果表明，CREV-PMN吞噬作用的缺陷是由于颗粒与PMN的C3b受体结合减少，而Fc受体系统保持完整。
