Qiu De-Lai, Akemann Walther, Chu Chun-Ping, Araki Rikita, Knöpfel Thomas
Laboratory for Neuronal Circuit Dynamics, RIKEN Brain Science Institute, Wako City, Saitama, Japan.
Neurosignals. 2008;16(4):289-99. doi: 10.1159/000123039. Epub 2008 Jul 18.
Interest in non-invasive methods for optical probing of neuronal electrical activity has been ongoing for several decades and methods for imaging the activity of single or multiple individual neurons in networks composed of thousands of neurons have been developed. Most widely used are techniques that use organic chemistry-based dyes as indicators of calcium and membrane potential. More recently a new generation of probes, genetically encoded fluorescent protein sensors, have emerged for use by physiologists studying the operation of neuronal circuits. In this review we describe the advance of these emerging optical techniques and compare them with more conventional approaches.
对用于神经元电活动光学探测的非侵入性方法的研究已经持续了几十年，并且已经开发出了用于对由数千个神经元组成的网络中的单个或多个神经元活动进行成像的方法。最广泛使用的技术是使用基于有机化学的染料作为钙和膜电位的指示剂。最近，新一代的探针，即基因编码荧光蛋白传感器，已出现供生理学家用于研究神经元回路的运作。在这篇综述中，我们描述了这些新兴光学技术的进展，并将它们与更传统的方法进行比较。
