• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

小鼠乳腺肿瘤细胞系在无血清培养基中的传代培养及小鼠乳腺肿瘤病毒的产生

Propagation of mouse mammary tumor cell lines and production of mouse mammary tumor virus in a serum-free medium.

作者信息

Bauer R F, Arthur L O, Fine D L

出版信息

In Vitro. 1976 Aug;12(8):558-63. doi: 10.1007/BF02797439.

DOI:10.1007/BF02797439
PMID:186396
Abstract

Five different mouse mammary tumor cell lines were propagated in a serum free medium. Evaluation of growth characteristics, including logarithmic growth, cell population increase, protein production and days to confluency, showed serum-free medium comparable to serum-containing medium. Mouse mammary tumor virus expression and production, in C3H and GR tumor cell lines, as determined by virus particle counting and RNA dependent DNA polymerase assays, subsequent to dexamethasone stimulation revealed equivalent to higher levels of virus in serum-free medium as compared to serum-containing medium.

摘要

五种不同的小鼠乳腺肿瘤细胞系在无血清培养基中传代培养。对生长特性的评估，包括对数生长期、细胞数量增加、蛋白质产生以及达到汇合所需天数，结果表明无血清培养基与含血清培养基相当。通过病毒颗粒计数和RNA依赖性DNA聚合酶测定法确定，在C3H和GR肿瘤细胞系中，地塞米松刺激后，无血清培养基中小鼠乳腺肿瘤病毒的表达和产生水平与含血清培养基相当或更高。

相似文献

1
Propagation of mouse mammary tumor cell lines and production of mouse mammary tumor virus in a serum-free medium.小鼠乳腺肿瘤细胞系在无血清培养基中的传代培养及小鼠乳腺肿瘤病毒的产生
In Vitro. 1976 Aug;12(8):558-63. doi: 10.1007/BF02797439.
2
Cell culture factors influencing in vitro expression of mouse mammary tumor virus.影响小鼠乳腺肿瘤病毒体外表达的细胞培养因素。
In Vitro. 1976 Oct;12(10):693-701. doi: 10.1007/BF02797473.
3
Demonstration of components of serum-free culture medium effecting maximum in vitro expression of mouse mammary tumor virus.无血清培养基成分对小鼠乳腺肿瘤病毒体外最大表达影响的证明。
In Vitro. 1978 Feb;14(2):218-6. doi: 10.1007/BF02618226.
4
In vitro system for production of mouse mammary tumor virus.用于生产小鼠乳腺肿瘤病毒的体外系统。
Appl Microbiol. 1974 Dec;28(6):1040-6. doi: 10.1128/am.28.6.1040-1046.1974.
5
Increased yield of mouse mammary tumor virus (MMTV) by cultivation of monolayer-derived mammary tumor cells in suspension.通过在悬浮液中培养单层来源的乳腺肿瘤细胞提高小鼠乳腺肿瘤病毒（MMTV）的产量。
In Vitro Cell Dev Biol. 1986 Sep;22(9):542-8. doi: 10.1007/BF02621141.
6
Large-scale production of mouse mammary tumor virus in the absence of endogenous murine leukemia virus.在无内源性鼠白血病病毒的情况下大规模生产小鼠乳腺肿瘤病毒。
Appl Environ Microbiol. 1979 Jan;37(1):148-58. doi: 10.1128/aem.37.1.148-158.1979.
7
Production of mouse mammary tumor virus by cultured cells in the absence and presence of hormones: assay by molecular hybridization.在有无激素情况下培养细胞产生小鼠乳腺肿瘤病毒：通过分子杂交进行测定
Virology. 1975 May;65(1):135-47. doi: 10.1016/0042-6822(75)90014-8.
8
Estimation of mouse mammary tumor virus gp52 abundance by protein A assay: a comparison of viral antigen expression on C3H and GR mammary tumor cells.通过蛋白A分析法估算小鼠乳腺肿瘤病毒gp52丰度：C3H和GR乳腺肿瘤细胞上病毒抗原表达的比较
Intervirology. 1981;15(2):111-20. doi: 10.1159/000149221.
9
Glucocorticoids stimulate the release of a viral tumor marker (MMTV gp52) while inhibiting tumor cell growth.糖皮质激素刺激一种病毒肿瘤标志物（小鼠乳腺肿瘤病毒糖蛋白52，MMTV gp52）的释放，同时抑制肿瘤细胞生长。
J Steroid Biochem. 1989 Apr;32(4):499-505. doi: 10.1016/0022-4731(89)90382-8.
10
Effects of interferon on the production of murine mammary tumor virus by mammary tumor cells in culture.干扰素对培养的乳腺肿瘤细胞产生小鼠乳腺肿瘤病毒的影响。
Virology. 1980 Apr 30;102(2):431-43. doi: 10.1016/0042-6822(80)90110-5.

引用本文的文献

1
Egg yolk lipoprotein, a new supplement for the growth of mammalian cells in serum-free medium : Egg yolk lipoprotein for serum-free growth of cells.蛋黄脂蛋白，一种无血清培养基中哺乳动物细胞生长的新型补充剂：无血清细胞生长用蛋黄脂蛋白。
Cytotechnology. 1988 Feb;1(2):159-69. doi: 10.1007/BF00146817.
2
Vitamin D-dependent suppression of endothelin-induced vascular smooth muscle cell proliferation through inhibition of CDK2 activity.维生素 D 依赖性抑制 CDK2 活性从而抑制内皮素诱导的血管平滑肌细胞增殖。
J Steroid Biochem Mol Biol. 2010 Feb 15;118(3):135-41. doi: 10.1016/j.jsbmb.2009.11.002. Epub 2009 Dec 2.
3
Expression of the vitamin d receptor is increased in the hypertrophic heart.

本文引用的文献

1
Measurement of cell growth in tissue culture with a phenol reagent (folin-ciocalteau).用酚试剂（福林-西奥卡泰奥试剂）测量组织培养中的细胞生长
Proc Soc Exp Biol Med. 1956 Feb;91(2):305-7. doi: 10.3181/00379727-91-22245.
2
Nutritional requirements of primary cultures. a neglected problem of modern biology.原代培养的营养需求。现代生物学中一个被忽视的问题。
In Vitro. 1974 Jul-Aug;10:119-29. doi: 10.1007/BF02615345.
3
Cation preferences for poly(rC)-oligo(dG)-directed DNA synthesis by RNA tumor viruses and human milk particulates.RNA肿瘤病毒和人乳微粒介导的聚（rC）-寡聚（dG）定向DNA合成的阳离子偏好性。
维生素D受体在肥厚型心脏中的表达增加。
Hypertension. 2008 Dec;52(6):1106-12. doi: 10.1161/HYPERTENSIONAHA.108.119602. Epub 2008 Oct 20.
4
Retinoic acid uses divergent mechanisms to activate or suppress mitogenesis in rat aortic smooth muscle cells.维甲酸通过不同机制激活或抑制大鼠主动脉平滑肌细胞的有丝分裂。
J Clin Invest. 1998 Aug 15;102(4):653-62. doi: 10.1172/JCI3483.
5
1,25(OH)2 vitamin D3, and retinoic acid antagonize endothelin-stimulated hypertrophy of neonatal rat cardiac myocytes.1,25-二羟维生素D3和视黄酸可拮抗内皮素刺激的新生大鼠心肌细胞肥大。
J Clin Invest. 1996 Apr 1;97(7):1577-88. doi: 10.1172/JCI118582.
6
Prolactin-deficient variants of GH3 rat pituitary tumor cells: linked expression of prolactin and another hormonally responsive protein in GH3 cells.GH3大鼠垂体瘤细胞的催乳素缺陷变体：GH3细胞中催乳素与另一种激素反应性蛋白的关联表达
Mol Cell Biol. 1982 Feb;2(2):179-89. doi: 10.1128/mcb.2.2.179-189.1982.
7
Thyroid or glucocorticoid hormone induces pre-growth-hormone mRNA and its probable nuclear precursor in rat pituitary cells.甲状腺激素或糖皮质激素可诱导大鼠垂体细胞中的前生长激素信使核糖核酸及其可能的核前体。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2230-4. doi: 10.1073/pnas.78.4.2230.
8
Regulated expression of chimaeric genes containing the 5'-flanking regions of human growth hormone-related genes in transiently transfected rat anterior pituitary tumor cells.在瞬时转染的大鼠垂体前叶肿瘤细胞中，含有人生长激素相关基因5'侧翼区的嵌合基因的调控表达。
Nucleic Acids Res. 1987 Feb 11;15(3):1297-309. doi: 10.1093/nar/15.3.1297.
9
Steroid inhibition of calcitriol-induced prolactin production in GH4C1 cells. Specificity and sensitivity.类固醇对生长激素4C1细胞中骨化三醇诱导的催乳素分泌的抑制作用。特异性与敏感性。
Biochem J. 1987 Jan 15;241(2):397-401. doi: 10.1042/bj2410397.
10
Selective regulation of trypsin gene expression by calcium and by glucose starvation in a rat exocrine pancreas cell line.
Proc Natl Acad Sci U S A. 1986 Jun;83(12):4292-6. doi: 10.1073/pnas.83.12.4292.
Cancer Res. 1974 Dec;34(12):3509-15.
4
Dexamethasone stimulation of murine mammary tumor virus expression: a tissue culture source of virus.地塞米松对鼠乳腺肿瘤病毒表达的刺激作用：病毒的一种组织培养来源。
Science. 1974 Apr 12;184(4133):158-60. doi: 10.1126/science.184.4133.158.
5
Tissue culture studies of mouse mammary tumor cells and associated viruses.小鼠乳腺肿瘤细胞及相关病毒的组织培养研究。
J Natl Cancer Inst. 1972 Nov;49(5):1321-32.
6
An established R3 mouse mammary tumor cell line; kinetics of mammary tumor virus (MTV) production.一种已建立的R3小鼠乳腺肿瘤细胞系；乳腺肿瘤病毒（MTV）产生的动力学。
Proc Soc Exp Biol Med. 1972 Jan;139(1):242-7. doi: 10.3181/00379727-139-36119.
7
Stimulation of mammary tumor virus production in a mouse mammary tumor cell line.小鼠乳腺肿瘤细胞系中乳腺肿瘤病毒产生的刺激作用。
Cancer Res. 1970 Apr;30(4):1109-17.
8
Studies on the cultivation of mammalian cell lines in a serum-free, chemically defined medium.关于在无血清、化学成分确定的培养基中培养哺乳动物细胞系的研究。
In Vitro. 1973 Sep-Oct;9(2):114-121. doi: 10.1007/BF02616009.
9
In vitro system for production of mouse mammary tumor virus.用于生产小鼠乳腺肿瘤病毒的体外系统。
Appl Microbiol. 1974 Dec;28(6):1040-6. doi: 10.1128/am.28.6.1040-1046.1974.
10
Enhanced production of mouse mammary tumor virus in dexamethasone-treated, 5-iododeoxyuridine-stimulated mammary tumor cell cultures.在地塞米松处理、5-碘脱氧尿苷刺激的乳腺肿瘤细胞培养物中，小鼠乳腺肿瘤病毒产量增加。
J Natl Cancer Inst. 1974 Jun;52(6):1881-6. doi: 10.1093/jnci/52.6.1881.