Louie Kathryn, Russo Gary J, Salkoff David B, Wellington Andrea, Zinsmaier Konrad E
Arizona Research Laboratories Division of Neurobiology, University of Arizona, Tucson, AZ 85721, USA.
Comp Biochem Physiol A Mol Integr Physiol. 2008 Oct;151(2):159-72. doi: 10.1016/j.cbpa.2008.06.023. Epub 2008 Jun 27.
The distribution of mitochondria is sensitive to physiological stresses and changes in metabolic demands. Consequently, it is important to carefully define the conditions facilitating live imaging of mitochondrial transport in dissected animal preparations. In this study, we examined Schneider's and the haemolymph-like solutions HL3 and HL6 for their suitability to image mitochondrial transport in motor axons of dissected Drosophila melanogaster larvae. Overall, mitochondrial transport kinetics in larval motor axons appeared similar among all three solutions. Unexpectedly, HL3 solution selectively increased the length of mitochondria in the context of the net-direction of transport. We also found that mitochondrial transport is sensitive to the extracellular Ca(2+) but not glutamate concentration. High concentrations of extracellular glutamate affected only the ratio between motile and stationary mitochondria. Our study offers a valuable overview of mitochondrial transport kinetics in larval motor axons of Drosophila under various conditions, guiding future studies genetically dissecting mechanisms of mitochondrial transport.
线粒体的分布对生理应激和代谢需求的变化很敏感。因此，仔细确定有利于在解剖动物标本中对线粒体运输进行实时成像的条件非常重要。在本研究中，我们检测了施奈德溶液以及类血淋巴溶液HL3和HL6，以评估它们对解剖的黑腹果蝇幼虫运动轴突中线粒体运输成像的适用性。总体而言，在所有三种溶液中，幼虫运动轴突中的线粒体运输动力学似乎相似。出乎意料的是，在运输的净方向上，HL3溶液选择性地增加了线粒体的长度。我们还发现，线粒体运输对细胞外Ca(2+)敏感，但对谷氨酸浓度不敏感。高浓度的细胞外谷氨酸仅影响活动线粒体与静止线粒体之间的比例。我们的研究对果蝇幼虫运动轴突在各种条件下的线粒体运输动力学提供了有价值的概述，为未来从遗传学角度剖析线粒体运输机制的研究提供了指导。
