Wakabayashi Y, Usui Y, Okunuki Y, Takeuchi M, Kezuka T, Iwasaki T, Goto H
Department of Ophthalmology, Hachiouji Medical Center of Tokyo Medical University and Department of Ophthalmology, Tokyo Medical University Hospital, Tokyo, Japan.
Diabet Med. 2008 Jul;25(7):875-7. doi: 10.1111/j.1464-5491.2008.02466.x.
To determine the intravitreous concentration of monokine induced by interferon-gamma (Mig) in patients with diabetic retinopathy (DR) and the relation between Mig and vascular endothelial growth factor (VEGF).
Vitreous samples were obtained at the time of vitrectomy from 41 eyes of 38 DR patients (30 with active DR and 11 with inactive DR) and from 15 eyes of 15 non-diabetic patients who had macular disease (control subjects). Human Mig and VEGF were quantified using a FACS Caliber flow cytometer.
The vitreous concentration of Mig was increased significantly in patients with both active and inactive DR [148.0 (31.6-997.2; median range) and 82.3 (25.7-347.7) pg/ml, respectively] compared with control subjects [21.0 (5.2-74.3) pg/ml; P < 0.0001 and P < 0.001, respectively]. In DR patients, a significant (P < 0.01) correlation was observed between vitreous concentrations of Mig and VEGF.
Our results suggest that Mig may play an important role in the pathogenesis of DR and works in consort with VEGF in the progression of pathological angiogenesis in DR.
测定糖尿病视网膜病变（DR）患者玻璃体内干扰素-γ诱导的单核细胞趋化蛋白（Mig）浓度，以及Mig与血管内皮生长因子（VEGF）之间的关系。
在玻璃体切割术时，从38例DR患者的41只眼中获取玻璃体样本（30例活动性DR患者和11例非活动性DR患者），并从15例患有黄斑疾病的非糖尿病患者（对照受试者）的15只眼中获取样本。使用FACS Caliber流式细胞仪对人Mig和VEGF进行定量分析。
与对照受试者[21.0（5.2 - 74.3）pg/ml]相比，活动性和非活动性DR患者的玻璃体内Mig浓度均显著升高[分别为148.0（31.6 - 997.2；中位数范围）和82.3（25.7 - 347.7）pg/ml；P < 0.0001和P < 0.001]。在DR患者中，观察到玻璃体内Mig和VEGF浓度之间存在显著相关性（P < 0.01）。
我们的结果表明，Mig可能在DR的发病机制中起重要作用，并在DR病理性血管生成的进展中与VEGF协同作用。
