Department of Ophthalmology, Hachiouji Medical Center, Tokyo Medical University, Tokyo, Japan.
Retina. 2011 Oct;31(9):1951-7. doi: 10.1097/IAE.0b013e31820d3cee.
To investigate whether concurrent hypertension affects vitreous cytokine levels in diabetic retinopathy.
Vitreous samples from 41 patients with diabetic retinopathy with or without concurrent hypertension, who underwent vitrectomy, were collected. Vitreous cytokine concentrations were simultaneously measured using flow cytometry. Patients were stratified according to hypertension or other clinical conditions, and the differences in vitreous levels of monocyte chemotactic protein 1, interleukin 8, vascular endothelial growth factor, interferon-inducible protein 10, and monokine induced by interferon gamma were examined.
Vitreous levels of monocyte chemotactic protein 1 and interleukin 8 were significantly (P < 0.05) higher in hypertensive patients than in nonhypertensive patients and were significantly (P < 0.05) higher in active diabetic retinopathy than in inactive diabetic retinopathy. Vitreous levels of vascular endothelial growth factor, interferon-inducible protein 10, and monokine induced by interferon gamma were not affected by the coexistence of hypertension. In multivariate models, active diabetic retinopathy (P = 0.004 and P = 0.007), systolic blood pressure (P = 0.039 and P = 0.041), and hypertension (P = 0.032 and P = 0.035) were significant and independent predictors for increased vitreous monocyte chemotactic protein 1 and interleukin 8 levels.
Both monocyte chemotactic protein 1 and interleukin 8 levels were elevated in the vitreous of patients with diabetic retinopathy and concurrent hypertension. These findings may help to explain the epidemiologic and clinical evidence that systemic hypertension exacerbates diabetic retinopathy.
研究并发高血压是否会影响糖尿病性视网膜病变患者的玻璃体细胞因子水平。
收集 41 例接受玻璃体切除术的伴或不伴并发高血压的糖尿病性视网膜病变患者的玻璃体样本。使用流式细胞术同时测量玻璃体细胞因子浓度。根据高血压或其他临床情况对患者进行分层，检查单核细胞趋化蛋白 1、白细胞介素 8、血管内皮生长因子、干扰素诱导蛋白 10 和干扰素γ诱导的单核因子在玻璃体中的差异。
高血压患者的玻璃体单核细胞趋化蛋白 1 和白细胞介素 8 水平明显高于非高血压患者（P<0.05），且活跃性糖尿病性视网膜病变患者的玻璃体单核细胞趋化蛋白 1 和白细胞介素 8 水平明显高于非活跃性糖尿病性视网膜病变患者（P<0.05）。血管内皮生长因子、干扰素诱导蛋白 10 和干扰素γ诱导的单核因子在玻璃体中的水平不受高血压共存的影响。在多变量模型中，活跃性糖尿病性视网膜病变（P=0.004 和 P=0.007）、收缩压（P=0.039 和 P=0.041）和高血压（P=0.032 和 P=0.035）是玻璃体单核细胞趋化蛋白 1 和白细胞介素 8 水平升高的显著和独立预测因子。
并发高血压的糖尿病性视网膜病变患者玻璃体中单核细胞趋化蛋白 1 和白细胞介素 8 水平升高。这些发现可能有助于解释高血压加重糖尿病性视网膜病变的流行病学和临床证据。
