Department of Ophthalmology, Hachiouji Medical Center of Tokyo Medical University, Tokyo, Japan.
Retina. 2010 Feb;30(2):339-44. doi: 10.1097/IAE.0b013e3181bd2f44.
The purpose of this study was to simultaneously measure the concentrations of multiple cytokines, including vascular endothelial growth factor, in the vitreous of patients with diabetic retinopathy and to examine their relation with clinical findings.
Vitreous samples from 46 eyes with diabetic retinopathy and 19 eyes with nondiabetic macular disease (controls) were used. Nine cytokines were simultaneously measured using a FACSCalibur flow cytometer.
Vascular endothelial growth factor, interleukin-8, monocyte chemotactic protein-1, interferon-inducible protein-10, and monokine induced by interferon-gamma were detected in the vitreous samples, and the concentrations were significantly (P < 0.001) higher in patients with diabetic retinopathy compared with control subjects. Vascular endothelial growth factor, interleukin-8, monocyte chemotactic protein-1, and monokine induced by interferon-gamma concentrations were significantly (P < 0.05) higher in active retinopathy than in inactive retinopathy. Furthermore, a significant (P < 0.01) positive correlation was observed between vascular endothelial growth factor concentration and interleukin-8, monocyte chemotactic protein-1, interferon-inducible protein-10, or monokine induced by interferon-gamma concentration in the vitreous.
Vascular endothelial growth factor, interleukin-8, monocyte chemotactic protein-1, interferon-inducible protein-10, and monokine induced by interferon-gamma were expressed at high levels locally in ocular tissues in diabetic retinopathy, and these cytokines may form a network and interact to impact the pathogenesis of the disease.
本研究旨在同时测量糖尿病性视网膜病变患者玻璃体中多种细胞因子（包括血管内皮生长因子）的浓度，并探讨其与临床发现的关系。
使用 46 只糖尿病性视网膜病变眼和 19 只非糖尿病性黄斑病变眼（对照组）的玻璃体样本。使用 FACSCalibur 流式细胞仪同时测量 9 种细胞因子。
在玻璃体样本中检测到血管内皮生长因子、白细胞介素-8、单核细胞趋化蛋白-1、干扰素诱导蛋白-10 和干扰素-γ诱导的单核因子，糖尿病性视网膜病变患者的浓度明显（P <0.001）高于对照组。血管内皮生长因子、白细胞介素-8、单核细胞趋化蛋白-1 和干扰素-γ诱导的单核因子在活动期视网膜病变中明显（P <0.05）高于非活动期视网膜病变。此外，在玻璃体中观察到血管内皮生长因子浓度与白细胞介素-8、单核细胞趋化蛋白-1、干扰素诱导蛋白-10 或干扰素-γ诱导的单核因子浓度之间存在显著（P <0.01）正相关。
在糖尿病性视网膜病变中，血管内皮生长因子、白细胞介素-8、单核细胞趋化蛋白-1、干扰素诱导蛋白-10 和干扰素-γ诱导的单核因子在眼部组织中高表达，这些细胞因子可能形成网络并相互作用，影响疾病的发病机制。
