Ward V K, Kalmakoff J
Department of Microbiology, University of Otago, P.O. Box 56, Dunedin, New Zealand.
Virology. 1987 Oct;160(2):507-10. doi: 10.1016/0042-6822(87)90027-4.
A physical map for the DNA genome of insect iridescent virus type 9 isolated from Wiseana spp. larvae [Lepidoptera: Hepialidae] was constructed using the restriction enzymes BamHl, EcoRl, and Pstl. Viral DNA was cloned into the plasmids pBR328 and pUC8 using Escherichia coli strains HB101 and JM83, respectively. The physical map for BamHl, EcoRl, and Pstl was constructed by multiple enzyme analysis and Southern hybridization of cloned viral DNA. Statistical analysis of restriction data by computer-aided linear modeling supported the physical map produced and indicated a total genome size of 192.5 kb. Due to the cyclic permutation of iridescent virus genomes the map is presented in a circular form.
利用限制性内切酶BamHl、EcoRl和Pstl构建了从怀蛾幼虫[鳞翅目：蝙蝠蛾科]中分离出的9型昆虫虹彩病毒DNA基因组的物理图谱。病毒DNA分别使用大肠杆菌菌株HB101和JM83克隆到质粒pBR328和pUC8中。通过对克隆的病毒DNA进行多酶分析和Southern杂交构建了BamHl、EcoRl和Pstl的物理图谱。通过计算机辅助线性建模对限制性数据进行统计分析，支持了所产生的物理图谱，并表明基因组总大小为192.5 kb。由于虹彩病毒基因组的环状排列，该图谱以圆形形式呈现。
