Thomsen D R, Stinski M F
Gene. 1981 Dec;16(1-3):207-16. doi: 10.1016/0378-1119(81)90077-9.
Restriction enzyme XbaI DNA fragments that represent 99% of the sequences from the long and short unique as well as the repeat sequences of the human cytomegalovirus (CMV) genome have been cloned into bacterial plasmid pACYC184. The viral DNA sequences associated with the recombinant plasmids were analyzed by restriction mapping and by hybridization to fragments of authentic viral DNA. The relationship of the cloned viral DNA fragments to the XbaI physical map of the viral genome is demonstrated. Even though large recombinant plasmids ranging from approx. 39 to 1.8 kb were isolated, most if not all of the viral DNA fragments were stable during propagation in Escherichia coli HB101.
代表人类巨细胞病毒（CMV）基因组的长独特序列、短独特序列以及重复序列中99%序列的限制性内切酶XbaI DNA片段已被克隆到细菌质粒pACYC184中。通过限制性图谱分析以及与真实病毒DNA片段杂交，对与重组质粒相关的病毒DNA序列进行了分析。展示了克隆的病毒DNA片段与病毒基因组XbaI物理图谱的关系。尽管分离出了大小约为39至1.8 kb的大型重组质粒，但在大肠杆菌HB101中繁殖期间，大多数（如果不是全部）病毒DNA片段都是稳定的。
