Auer I O, Grosch L, Hardörfer C, Röder A
Department of Medicine, Medical School, University of Würzburg, FRG.
Gut. 1988 Dec;29(12):1639-47. doi: 10.1136/gut.29.12.1639.
Serum antibodies cytotoxic to the colon cancer cell line RPMI 4788 were studied in 42 patients with ulcerative colitis, 61 patients with Crohn's disease, 27 patients with other inflammatory diseases (disease-controls) and 22 healthy controls. Cytotoxicity of antibodies towards RPMI 4788 was studied by means of a chromium release assay using peripheral blood mononuclear leucocytes of healthy subjects as effector cells. Using a four hour antibody dependent cell mediated cytotoxicity assay sera from 29% of ulcerative colitis patients contained antibodies cytotoxic for the target, while only 3% of the Crohn's patients and 6% of the disease controls and non of the healthy controls were positive. When an 18 hour assay was applied, however, not only 40% of ulcerative colitis patients, but also 14% of Crohn's patients and 21% of disease controls were found positive. The reactive antibody in the four hour assay was mainly of the IgG class, which points at a classical antibody dependent cell mediated cytotoxicity mechanism. In the 18 hour cytotoxic assay IgG and particularly IgM antibodies were found to be reactive. This suggests that in the latter case other cellular cytotoxic mechanism might be involved. There was a significant inverse correlation between the appearance of the ulcerative colitis restricted IgG-anticolon epithelial cell antibodies (four hour assay) and the disease activity (p less than 0.01). Absorption studies showed that the reactive antigen is not specific for ulcerative colitis colonic tissue, but is similarly found in Crohn's bowel tissue, and to a lower extent in normal bowel, liver and kidney. The reactive antigen, however, could not be detected in brain and lymphoblastoid cells.
在42例溃疡性结肠炎患者、61例克罗恩病患者、27例其他炎症性疾病患者（疾病对照）和22例健康对照中，研究了对结肠癌细胞系RPMI 4788具有细胞毒性的血清抗体。采用以健康受试者外周血单个核白细胞作为效应细胞的铬释放试验，研究抗体对RPMI 4788的细胞毒性。使用四小时抗体依赖性细胞介导的细胞毒性试验，29%的溃疡性结肠炎患者血清中含有对靶细胞具有细胞毒性的抗体，而只有3%的克罗恩病患者、6%的疾病对照患者以及无健康对照患者呈阳性。然而，当采用18小时试验时，不仅40%的溃疡性结肠炎患者呈阳性，而且14%的克罗恩病患者和21%的疾病对照患者也呈阳性。四小时试验中的反应性抗体主要为IgG类，这表明存在经典的抗体依赖性细胞介导的细胞毒性机制。在18小时细胞毒性试验中，发现IgG尤其是IgM抗体具有反应性。这表明在后一种情况下可能涉及其他细胞毒性机制。溃疡性结肠炎特异性IgG抗结肠上皮细胞抗体的出现（四小时试验）与疾病活动度之间存在显著负相关（p小于0.01）。吸收研究表明，反应性抗原并非溃疡性结肠炎结肠组织所特有，在克罗恩病肠组织中也同样存在，在正常肠、肝和肾中含量较低。然而，在脑和淋巴母细胞中未检测到反应性抗原。
