Fadly A M, Witter R L, Smith E J, Silva R F, Reed W M, Hoerr F J, Putnam M R
USDA-Agricultural Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI, USA.
Avian Pathol. 1996 Mar;25(1):35-47. doi: 10.1080/03079459608419118.
Gross and microscopic examinations of affected tissues from chickens of two commercial broiler breeder flocks aged 27 and 31 weeks revealed lesions of visceral lymphomas with bursal involvement in some chickens. Reticuloendotheliosis virus (REV), but not avian leukosis virus (ALV), was isolated from blood of affected chickens. Furthermore, DNA extracted from tumours tested positive for REV, but not for ALV or Marek's disease virus by polymerase chain reaction (PCR) test. Attempts to determine the source of REV infection included testing a commercial fowlpox (FP) vaccine used to immunize flocks at 7 days of age. Chicken-embryo fibroblasts inoculated with the FP vaccine tested positive for REV by PCR and immunofluorescent tests. REV was also isolated from plasma of pathogen-free chickens experimentally inoculated with FP vaccine at hatch; two of eight (25%) inoculated chickens developed lymphomas by 34 weeks of age. Antigenic characterization of REV isolated from commercial broiler breeder chickens and from FP vaccine, using monoclonal antibodies, revealed that both isolates belong to subtype 3 of REV. The data represent the first report of an outbreak of REV-induced lymphomas in commercial chickens. The data also indicate that the source of REV infection is an REV-contaminated commercial FP vaccine.
对两个商业肉种鸡群（分别为27周龄和31周龄）中患病鸡的受影响组织进行大体和显微镜检查，发现一些鸡存在内脏淋巴瘤病变且法氏囊受累。从患病鸡的血液中分离出了网状内皮组织增生症病毒（REV），而非禽白血病病毒（ALV）。此外，通过聚合酶链反应（PCR）检测，从肿瘤中提取的DNA对REV呈阳性反应，但对ALV或马立克氏病病毒呈阴性。确定REV感染源的尝试包括检测一种用于7日龄鸡群免疫的商业鸡痘（FP）疫苗。接种FP疫苗的鸡胚成纤维细胞经PCR和免疫荧光检测对REV呈阳性反应。在孵化时用FP疫苗对无特定病原体鸡进行实验性接种，也从其血浆中分离出了REV；8只接种鸡中有2只（25%）在34周龄时发生了淋巴瘤。使用单克隆抗体对从商业肉种鸡和FP疫苗中分离出的REV进行抗原特性分析，结果表明这两种分离株均属于REV的3型。这些数据代表了商业鸡中REV诱导淋巴瘤爆发的首次报告。数据还表明，REV感染源是一种受REV污染的商业FP疫苗。
