China Animal Health and Epidemiology Center, Qingdao, China.
China Institute of Veterinary Drug Control, Beijing, China.
PLoS One. 2019 Apr 22;14(4):e0213978. doi: 10.1371/journal.pone.0213978. eCollection 2019.
Reticuloendotheliosis virus (REV) is the most frequent exogenous virus that contaminates attenuated vaccines. Therefore, it is extremely important to select REV-free specific-pathogen-free (SPF) chicken embryos. Generally, REV infection is assessed by detecting REV antibodies in SPF chickens. This present study seeks to evaluate REV infection by replacing serum antibody detection with yolk antibody detection. A cohort of 40 nineteen-week-old SPF chickens were artificially inoculated with REV, with 32 SPF chickens raised in another isolation environment served as a blank control. Eggs and serum from 23-week-old chickens were sampled, and yolks were diluted separately to ratios of 1:150, 1:200, 1:300 and 1:400, which were detected together with serum. We found that the yolk antibody detection findings at a dilution of 1:300 had the highest coincidence rate compared with that based on serum antibody measurements. At a dilution ratio of 1:300 for yolk antibody, 72 chickens were continuously observed for 10 weeks from 25- to 34-weeks-old. Our findings were based on serum antibody or yolk antibody detection, and the evaluation results were completely consistent. Therefore, all serum antibody-positive chickens were yolk antibody-positive, and vice versa. Accordingly, vaccine producers can estimate REV cleanliness in a poultry farm by sampling yolk antibody titers.
网状内皮组织增生病病毒（REV）是最常见的污染弱毒疫苗的外源病毒。因此，选择无 REV 的特定病原（SPF）鸡胚是极其重要的。通常，通过检测 SPF 鸡中的 REV 抗体来评估 REV 感染。本研究旨在通过用卵黄抗体检测代替血清抗体检测来评估 REV 感染。一组 40 只 19 周龄的 SPF 鸡被人工接种 REV，另一组 32 只 SPF 鸡在另一个隔离环境中饲养作为空白对照。采集 23 周龄鸡的鸡蛋和血清，并将蛋黄分别以 1:150、1:200、1:300 和 1:400 的比例稀释，与血清一起检测。我们发现，与基于血清抗体测量相比，在 1:300 的稀释度下，卵黄抗体检测结果的符合率最高。在 1:300 的稀释度下进行卵黄抗体检测时，从 25 周到 34 周龄，连续观察了 72 只鸡 10 周。我们的发现基于血清抗体或卵黄抗体检测，评估结果完全一致。因此，所有血清抗体阳性的鸡都是卵黄抗体阳性，反之亦然。因此，疫苗生产商可以通过采样卵黄抗体滴度来估计家禽养殖场的 REV 清洁度。
