USDA-ARS, Food Animal Protection Research Laboratory, College Station, Texas, USA.
Avian Pathol. 1996 Dec;25(4):737-49. doi: 10.1080/03079459608419178.
The prophylactic treatment of neonatal broiler chicks with lymphokines derived from S. enteritidis-immurazed chickens (SE-ILK) was evaluated for its effect on the birds' resistance to an experimental infection S. enterica ser. gallinarum (SG). On the day of hatch, chicks were injected intraperitoneally with either SE-ILK, control non-immune lymphokines (NILK), or were left untreated. Thirty minutes later, all chicks were orally gavaged with either 10(4) colony forming units (CFU) or 10(6) CFU SG. The chicks were observed twice daily for 10 days for morbidity and mortality. Chicks that died during the experiment had their livers cultured for SG. The survivors were killed and their livers, spleens and caecal tonsils cultured for SG. The prophylactic treatment of chickens with SE-ILK induced significant protection against extraintestinal SG infection when compared to the NILK-treated or non-treated controls as evidenced by: (1) a significant reduction (P< 0.005) in the mortality of chicks challenged with either 10(4) and 10(6) CFU SG; (2) an increased average weight gains of chicks challenged with either 10(4) and 10(6) CFU SG; and (3) a significant (P< 0.001) reduction in the number of chicks with organs culture-positive for SG. The results suggest that the prophylactic administration of SE-ILK can confer non-specific protection to chicks against a pathogenic species of Salmonella resulting in reduced morbidity, mortality, and organ infectivity caused by SG infections of broiler chicks, while enhancing performance during the first 10 days of Ufe.
用来源于肠炎沙门氏菌免疫鸡的淋巴因子（SE-ILK）对初生肉仔鸡进行预防性治疗，以评价其对鸡抵抗实验性肠炎沙门氏菌血清型（SG）感染的影响。孵出当天，雏鸡经腹腔注射 SE-ILK、对照非免疫淋巴因子（NILK）或不处理。30 分钟后，所有雏鸡均经口灌胃 10（4）或 10（6）CFU SG。观察雏鸡 10 天，每天两次，记录发病率和死亡率。试验期间死亡的雏鸡进行肝脏 SG 培养。幸存者被处死，其肝脏、脾脏和盲肠扁桃体进行 SG 培养。与 NILK 处理组或未处理对照组相比，用 SE-ILK 对鸡进行预防性治疗可显著抵抗肠外 SG 感染，证据如下：（1）用 10（4）和 10（6）CFU SG 攻毒的雏鸡死亡率显著降低（P<0.005）；（2）用 10（4）和 10（6）CFU SG 攻毒的雏鸡平均增重增加；（3）SG 器官培养阳性的雏鸡数量显著减少（P<0.001）。结果表明，预防性给予 SE-ILK 可赋予雏鸡非特异性保护，降低由 SG 感染引起的肉仔鸡发病率、死亡率和器官感染性，同时增强其生命早期 10 天的性能。
