血液中地西泮和N-去甲基地西泮的定量高效液相色谱法。
Quantitative high-performance liquid chromatography of diazepam and N-desmethyldiazepam in blood.
作者信息
Bugge A
出版信息
J Chromatogr. 1976 Nov 17;128(1):111-6. doi: 10.1016/s0021-9673(00)84036-4.
PMID:186471
Abstract
High-performance liquid chromatography on porous silica has been employed to determine diazepam and N-desmethyldiazepam in human blood. For forensic purposes, 1.0 ml of blood is sufficient for a quantitative determination of the benzodiazepines in concentrations above 100 ng/ml. In cases where lower levels, 25-100 ng/ml, are of interest, 2.0 ml of blood together with a somewhat more elaborate extraction procedure are necessary.
摘要
已采用在多孔硅胶上的高效液相色谱法测定人血中的地西泮和N-去甲基地西泮。出于法医目的，1.0毫升血液足以对浓度高于100纳克/毫升的苯二氮䓬类药物进行定量测定。对于浓度在25 - 100纳克/毫升的较低水平情况，需要2.0毫升血液以及更为精细的提取程序。
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