Gomes Niele de Matos, Rezende Claudia de Moraes, Fontes Silvia Paredes, Hovell Ana Maria Celestino, Landgraf Richardt Gama, Matheus Maria Eline, Pinto Angelo da Cunha, Fernandes Patricia Dias
Programa de Desenvolvimento de Fármacos, ICB, Universidade Federal do Rio de Janeiro, Caixa Postal 68016, CEP 21944-970, Brazil.
J Ethnopharmacol. 2008 Sep 2;119(1):179-84. doi: 10.1016/j.jep.2008.06.033. Epub 2008 Jul 5.
The aim of this study was to investigate the effect of chronic treatment with C. multijuga oil on Ehrlich tumor evolution. C. multijuga was fractionated in a KOH impregnated silica gel column chromatography to give three distinct fractions, i.e., hexanic, chloroformic, and methanolic, mainly composed by hydrocarbon sesquiterpenes, oxygenated sesquiterpenes and acidic diterpenes, respectively. Results demonstrated that the C. multijuga oil, the hexanic, and chloroformic fractions did not develop toxic effects. The oil, hexanic and chloroformic fractions (doses varying between 100 and 200mg/kg) showed antineoplasic properties against Ehrlich ascitic tumor (EAT) and solid tumor during 10 consecutive days of treatment inhibiting ascitic tumor cell number, reverting medulla and blood cell counts to values similar to control group, and inhibiting the increase on several inflammatory mediators (total protein, PGE(2), nitric oxide, and TNF) on ascitic fluid. The treatment also inhibited the increase in paw volume on tumor-inoculated mice. In conclusion, C. multijuga as well as its fractions demonstrated antineoplasic effect even after oral administration confirming its use by traditional medicine.
本研究的目的是调查多花菊油长期治疗对艾氏肿瘤演变的影响。多花菊通过在氢氧化钾浸渍硅胶柱色谱中进行分馏，得到三个不同的馏分，即己烷馏分、氯仿馏分和甲醇馏分，它们分别主要由烃倍半萜、氧化倍半萜和酸性二萜组成。结果表明，多花菊油、己烷馏分和氯仿馏分未产生毒性作用。在连续10天的治疗期间，该油、己烷馏分和氯仿馏分（剂量在100至200mg/kg之间变化）对艾氏腹水瘤（EAT）和实体瘤显示出抗肿瘤特性，抑制腹水瘤细胞数量，使骨髓和血细胞计数恢复到与对照组相似的值，并抑制腹水中几种炎症介质（总蛋白、前列腺素E2、一氧化氮和肿瘤坏死因子）的增加。该治疗还抑制了接种肿瘤小鼠爪体积的增加。总之，多花菊及其馏分即使在口服给药后也显示出抗肿瘤作用，证实了传统医学对其的使用。
