Conti L, Di Marzio P, Gessani S, Borghi P, Varano B, Dieffenbach C, Belardelli F
Istituto Superiore di Sanitá, Department of Virology, Viale Regina Elena 299, Rome, Italy.
Arch Gerontol Geriatr. 1992;15 Suppl 1:123-8. doi: 10.1016/s0167-4943(05)80012-3.
The expression of interferon (IFN)-beta gene and its modulation during in vitro "aging" was studied in unstimulated peritoneal macrophages (PM) explanted from lipopolysaccharide (LPS)-responsive (Lps(h)) and LPS-hyporesponsive (Lps(d)) mice. A strong direct correlation between the LPS response of PM and their capacity to express low levels of IFN-beta was found. Moreover, the decay of antiviral state during in vitro cultivation was correlated with the turnover of IFN-beta mRNA. In the light of the multiple biologic effect of IFN, the constitutive expression of IFN-beta gene in PM can play a crucial role not only in the restriction of viral replication, but also in the modulation of cell differentiation and immune response.
在从脂多糖（LPS）反应性（Lps(h)）和LPS低反应性（Lps(d)）小鼠分离出的未刺激腹膜巨噬细胞（PM）中，研究了干扰素（IFN）-β基因的表达及其在体外“老化”过程中的调节。发现PM的LPS反应与其表达低水平IFN-β的能力之间存在很强的直接相关性。此外，体外培养期间抗病毒状态的衰减与IFN-β mRNA的周转相关。鉴于IFN的多种生物学效应，PM中IFN-β基因的组成型表达不仅在限制病毒复制中起关键作用，而且在细胞分化和免疫反应的调节中也起关键作用。
