中国南方农村地区的猪戊型肝炎病毒：恒河猴（猕猴）的基因特征及实验性感染
Swine hepatitis E virus in rural southern China: genetic characterization and experimental infection in rhesus monkeys (Macaca mulatta).
作者信息
Ji Yanli, Zhu Yonghong, Liang Jingrui, Wei Xianfei, Yang Xi, Wang Ling, Li Lingjun, Chang Yibin, Tang Ronglan, Zhuang Hui
机构信息
Department of Microbiology, Peking University Health Science Center, 38 Xuanyuan Road, Beijing 100083, People's Republic of China.
出版信息
J Gastroenterol. 2008;43(7):565-70. doi: 10.1007/s00535-008-2196-3. Epub 2008 Jul 23.
BACKGROUND
In rural areas of southern China, where hepatitis E is endemic, residents generally rear pigs in pigsties near their houses. The study was conducted to assess the possibility that hepatitis E virus (HEV) infections in this region are acquired primarily through contact with swine.
METHODS
One hundred twenty swine fecal samples collected from pigsties located in eight rural communities of southern China were tested for HEV RNA. The swine HEV isolates were analyzed genetically and were experimentally inoculated into rhesus monkeys to determine the potential risk of cross-species infection.
RESULTS
Twenty-nine of the 120 swine fecal samples were positive for HEV RNA. The nucleotide sequences of these swine HEV strains shared 85%-99% identities with the local human genotype 4 isolates and belonged to two subgroups of genotype 4. Importantly, swine HEV strains representing both subgroups induced hepatitis in rhesus monkeys by inoculation with the virus, evidenced by elevated serum alanine transaminase (ALT), viremia, fecal viral shedding, anti-HEV seroconversion, and liver histopathological changes.
CONCLUSIONS
Swine may be the principal reservoir for human HEV infection in rural southern China.
背景
在中国南方农村地区，戊型肝炎呈地方性流行，当地居民通常在房屋附近的猪圈养猪。本研究旨在评估该地区戊型肝炎病毒（HEV）感染主要通过与猪接触而获得的可能性。
方法
对从中国南方八个农村社区的猪圈采集的120份猪粪便样本进行HEV RNA检测。对猪源HEV分离株进行基因分析，并将其实验性接种到恒河猴体内，以确定跨物种感染的潜在风险。
结果
120份猪粪便样本中有29份HEV RNA呈阳性。这些猪源HEV毒株的核苷酸序列与当地人类4型基因型分离株的同源性为85%-99%，属于4型基因型的两个亚组。重要的是，代表两个亚组的猪源HEV毒株通过接种病毒在恒河猴中诱发了肝炎，血清丙氨酸转氨酶（ALT）升高、病毒血症、粪便病毒排出、抗HEV血清转化以及肝脏组织病理学变化均证明了这一点。
结论
在中国南方农村地区，猪可能是人类感染HEV的主要宿主。
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