利用免疫荧光法鉴定麻疹病毒感染。
Use of immunofluorescence to identify measles virus infections.
作者信息
Minnich L L, Goodenough F, Ray C G
机构信息
Department of Pathology, University of Arizona, Tucson 85724.
出版信息
J Clin Microbiol. 1991 Jun;29(6):1148-50. doi: 10.1128/jcm.29.6.1148-1150.1991.
Monoclonal antibody to measles virus was used successfully to identify measles virus antigen directly in clinical specimens, as well as in cell cultures. Pooled nasopharyngeal-throat swab specimens had a higher yield than throat swabs or urine samples for virus detection. Use of A549 cell cultures in the spin amplification vial assay proved to be highly efficient, allowing virus recognition within 1 to 2 days of inoculation. A combination of appropriately collected specimens, which includes a nasopharyngeal-throat swab, direct antigen detection with monoclonal antibody to measles in an indirect immunofluorescence system, and the spin amplification vial assay using A549 cells provides a sensitive and rapid system for isolation and/or identification of measles virus infections.
麻疹病毒单克隆抗体已成功用于直接鉴定临床标本以及细胞培养物中的麻疹病毒抗原。用于病毒检测时，合并的鼻咽拭子标本比咽拭子或尿液样本具有更高的检出率。在旋转扩增管试验中使用A549细胞培养物被证明是高效的，接种后1至2天内即可识别病毒。适当采集的标本（包括鼻咽拭子）、在间接免疫荧光系统中使用麻疹单克隆抗体进行直接抗原检测以及使用A549细胞的旋转扩增管试验相结合，可以提供一个敏感且快速的系统，用于分离和/或鉴定麻疹病毒感染。
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