Fulton R E, Middleton P J
J Pediatr. 1975 Jan;86(1):17-22. doi: 10.1016/s0022-3476(75)80698-6.
Nasopharyngeal cells of 24 patients diagnosed as being in the prodromal phase or with established measles were examined by indirect immunofluorescence microscopy for the presence of measles antigen. Eighteen patients were positive for measles antigen by this technique. In ten of the 18 patients it was necessary to remove masking globulins from the antigen-containing cells prior to staining. Results obtained by immunofluorescence were supported by cytologic and serologic findings. No virus isolations were achieved. Immunofluorescence microscopy with elution of adherent globulins offers the most rapid and sensitive method for the laboratory diagnosis of measles.
对24例被诊断为处于麻疹前驱期或已确诊麻疹的患者的鼻咽细胞进行间接免疫荧光显微镜检查，以检测麻疹抗原的存在。通过该技术，18例患者的麻疹抗原呈阳性。在这18例患者中的10例中，在染色前有必要从含抗原的细胞中去除封闭球蛋白。免疫荧光结果得到了细胞学和血清学检查结果的支持。未分离到病毒。洗脱黏附球蛋白的免疫荧光显微镜检查为麻疹的实验室诊断提供了最快速、灵敏的方法。
