通过旋转扩增空斑培养和单克隆抗体检测法从临床标本中检测肠道病毒。
Detection of enteroviruses from clinical specimens by spin amplification shell vial culture and monoclonal antibody assay.
作者信息
Klespies S L, Cebula D E, Kelley C L, Galehouse D, Maurer C C
机构信息
Department of Pathology and Laboratory Medicine, Children's Hospital Medical Center of Akron, Ohio 44308, USA.
出版信息
J Clin Microbiol. 1996 Jun;34(6):1465-7. doi: 10.1128/jcm.34.6.1465-1467.1996.
Abstract
Conventional tube cell culture was compared with a 72-h, spin-amplified shell vial indirect immunofluorescence assay for the detection of enterovirus from clinical specimens. The sensitivity for the shell vial assay after resolution of discrepant results were 93 and 100%, respectively. The shell vial assay detected 93% of the positive cultures within 72 h of incubation while conventional tube culture detected only 51% of the positive cultures within the same time interval. The data suggest that a spin-amplified shell vial indirect immunofluorescence assay may be useful for the detection of enterovirus from clinical specimens.
摘要
将传统的试管细胞培养法与一种用于从临床标本中检测肠道病毒的72小时旋转扩增空斑试验间接免疫荧光测定法进行了比较。在解决结果差异后，空斑试验的灵敏度分别为93%和100%。空斑试验在培养72小时内检测出93%的阳性培养物，而传统试管培养在相同时间间隔内仅检测出51%的阳性培养物。数据表明，旋转扩增空斑试验间接免疫荧光测定法可能有助于从临床标本中检测肠道病毒。
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