在疫苗接种者尿液样本中检测麻疹病毒RNA
Detection of measles virus RNA in urine specimens from vaccine recipients.
作者信息
Rota P A, Khan A S, Durigon E, Yuran T, Villamarzo Y S, Bellini W J
机构信息
Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
出版信息
J Clin Microbiol. 1995 Sep;33(9):2485-8. doi: 10.1128/jcm.33.9.2485-2488.1995.
Abstract
Analysis of urine specimens by using reverse transcriptase-PCR was evaluated as a rapid assay to identify individuals infected with measles virus. For the study, daily urine samples were obtained from either 15-month-old children or young adults following measles immunization. Overall, measles virus RNA was detected in 10 of 12 children during the 2-week sampling period. In some cases, measles virus RNA was detected as early as 1 day or as late as 14 days after vaccination. Measles virus RNA was also detected in the urine samples from all four of the young adults between 1 and 13 days after vaccination. This assay will enable continued studies of the shedding and transmission of measles virus and, it is hoped, will provide a rapid means to identify measles infection, especially in mild or asymptomatic cases.
摘要
采用逆转录-聚合酶链反应对尿液标本进行分析，以此作为一种快速检测方法来鉴定感染麻疹病毒的个体。在这项研究中，对15个月大的儿童或年轻成人在接种麻疹疫苗后每日采集尿液样本。总体而言，在为期2周的采样期内，12名儿童中有10名检测到麻疹病毒RNA。在某些情况下，接种疫苗后1天或14天就检测到了麻疹病毒RNA。在4名年轻成人接种疫苗后1至13天的尿液样本中也检测到了麻疹病毒RNA。该检测方法将有助于对麻疹病毒的排出和传播进行持续研究，并且有望提供一种快速手段来鉴定麻疹感染，尤其是在轻症或无症状病例中。
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