Li Yu-feng, He Qiao-hua, Zhang Han-wang, Zhu Gui-jin, Hu Juan, Liu Qun, Ren Xin-ling, Wei Yu-lan
Reproductive Center, Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Zhonghua Nan Ke Xue. 2008 Jun;14(6):498-502.
To examine the influence of cryoloop on the spindle and chromosome configurations of human oocytes cryopreserved in the germinal vesicle (GV) and metaphase II (M II) stages, as well as on the survival rate and potential for in vitro maturation (IVM).
GV oocytes were randomly assigned into a control group (matured in vitro into the M II stage), a GV cryopreserved group (cryopreserved in the GV stage and then matured in vitro), and an M II cryopreserved group (matured in vitro and cryopreserved in the M II stage). After cryopreservation and IVM, immunostaining of the tubulin and chromatin was performed followed by visualization using laser scanning confocal microscopy (LSCM).
A significantly higher survival rate was observed in the GV cryopreserved group than in the M II , but the maturation rate showed no significant difference between the GV cryopreserved group and the control (P > 0.05). Compared with the control group, there was a statistically significant decrease in the rates of normal meiotic spindles and chromosomes in the GV cryopreserved group (P < 0.05). A significantly lower rate of normal spindles was noted in the M II cryopreserved group than in the control, but no statistical difference was shown in the rate of normal meiotic chromosomes between the two groups (P > 0.05).
Cryopreservation by cryoloop has a damaging effect on the spindle and chromosome of human oocytes in the GV and M II stages.
探讨冷冻环对处于生发泡（GV）期和减数分裂中期II（M II）期的冷冻保存的人类卵母细胞纺锤体和染色体构型的影响，以及对存活率和体外成熟（IVM）潜能的影响。
将GV期卵母细胞随机分为对照组（体外成熟至M II期）、GV期冷冻保存组（在GV期冷冻保存，然后体外成熟）和M II期冷冻保存组（体外成熟并在M II期冷冻保存）。冷冻保存和IVM后，进行微管蛋白和染色质的免疫染色，然后使用激光扫描共聚焦显微镜（LSCM）进行观察。
GV期冷冻保存组的存活率显著高于M II期冷冻保存组，但GV期冷冻保存组与对照组之间的成熟率无显著差异（P>0.05）。与对照组相比，GV期冷冻保存组正常减数分裂纺锤体和染色体的比例有统计学意义的下降（P<0.05）。M II期冷冻保存组正常纺锤体的比例显著低于对照组，但两组间正常减数分裂染色体的比例无统计学差异（P>0.05）。
冷冻环冷冻保存对GV期和M II期人类卵母细胞的纺锤体和染色体有损伤作用。
