Hu Yong-Mei, Liu Chuan, Cheng Ka-Wing, Sung Herman H-Y, Williams Lan D, Yang Zhong-Lin, Ye Wen-Cai
Department of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing 210009, PR China.
Phytochemistry. 2008 Sep;69(12):2367-73. doi: 10.1016/j.phytochem.2008.05.023. Epub 2008 Jul 21.
Chemical investigation of rhizomes of Homalomena occulta (Lours) resulted in isolation and identification of two sesquiterpenoids (6,7), and one daucane ester 8, together with five known sesquiterpenoids, oplodiol, oplopanone, homalomenol C, bullatantriol, and 1beta,4beta,7alpha-trihydroxyeudesmane. Their structures were elucidated using 1D and 2D NMR spectroscopic and X-ray analyses. The chloroform extract of this plant and compounds 1-7 were tested in vitro for their activities in stimulating osteoblast (OB) proliferation, differentiation and mineralization. Compounds 1-4 had a stimulative effect on significantly proliferation and differentiation of culture osteoblasts, while the chloroform extract and 1 significantly stimulated mineralization of cultured osteoblasts in vitro.
对千年健（Homalomena occulta (Lours)）根茎进行化学研究，结果分离并鉴定出两种倍半萜类化合物（6,7）和一种胡萝卜烷酯8，以及五种已知的倍半萜类化合物，蛇麻脂二醇、蛇麻脂酮、千年健醇C、波拉特三醇和1β,4β,7α-三羟基桉叶烷。通过一维和二维核磁共振光谱以及X射线分析阐明了它们的结构。对该植物的氯仿提取物以及化合物1 - 7进行了体外测试，以检测它们在刺激成骨细胞（OB）增殖、分化和矿化方面的活性。化合物1 - 4对培养的成骨细胞的增殖和分化有显著的刺激作用，而氯仿提取物和1在体外显著刺激了培养的成骨细胞的矿化。
