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对从热带地区归来的旅行者进行登革病毒的聚合酶链反应检测。

Pan-dengue virus detection by PCR for travelers returning from the tropics.

作者信息

Dumoulin Alexis, Marti Hanspeter, Panning Marcus, Hatz Christoph, Hirsch Hans H

机构信息

Department of Biomedicine, Division of Diagnostics, Institute for Medical Microbiology, University of Basel, Basel, Switzerland.

出版信息

J Clin Microbiol. 2008 Sep;46(9):3104-6. doi: 10.1128/JCM.01294-08. Epub 2008 Jul 23.

Abstract

We performed dengue virus (DENV) serology and quantitative real-time pan-DENV reverse transcription-PCR (RT-PCR) on 186 sera of 171 patients returning from the tropics. DENV loads significantly decreased with increasing times of disease and were higher in immunoglobulin M-negative samples. In the first week of disease, pan-DENV RT-PCR is the test of choice.

摘要

我们对171名从热带地区归来患者的186份血清样本进行了登革病毒(DENV)血清学检测及定量实时全登革病毒逆转录聚合酶链反应(RT-PCR)检测。登革病毒载量随病程延长显著下降,且在免疫球蛋白M阴性样本中更高。在病程第一周,全登革病毒RT-PCR是首选检测方法。

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