Lee Hyoung-Joo, Kang Min-Jeong, Lee Eun-Young, Cho Sang Yun, Kim Hoguen, Paik Young-Ki
Department of Biochemistry, Yonsei Proteome Research Center and Biomedical Proteome Research Center, Yonsei University, Seoul, Korea.
Proteomics. 2008 Aug;8(16):3371-81. doi: 10.1002/pmic.200800111.
A peptide-based 2-D liquid phase fractionation (PF2D) system was used in a quantitative proteomic analysis of hepatocellular carcinoma. 2-D liquid maps of peptide specimens showed better resolution than those of proteins, leading to the identification of differentially expressed proteins. Peptide-based PF2D gave well-matched theoretical and experimental pI values and was proven to be a very efficient and versatile analytical tool for both large-scale profiling and quantification of phosphoproteins in disease biomarker discovery.
一种基于肽的二维液相分级分离（PF2D）系统被用于肝细胞癌的定量蛋白质组分析。肽样本的二维液相图谱显示出比蛋白质图谱更好的分辨率，从而能够鉴定差异表达的蛋白质。基于肽的PF2D给出了匹配良好的理论和实验pI值，并且被证明是一种非常高效且通用的分析工具，可用于疾病生物标志物发现中磷酸化蛋白质的大规模分析和定量。
