Ravalason Holy, Jan Gwénaël, Mollé Daniel, Pasco Maryvonne, Coutinho Pedro M, Lapierre Catherine, Pollet Brigitte, Bertaud Frédérique, Petit-Conil Michel, Grisel Sacha, Sigoillot Jean-Claude, Asther Marcel, Herpoël-Gimbert Isabelle
INRA, UMR1163, Biotechnologie des Champignons Filamenteux, 13000, Marseille, France.
Appl Microbiol Biotechnol. 2008 Sep;80(4):719-33. doi: 10.1007/s00253-008-1596-x. Epub 2008 Jul 25.
Proteomic analysis was performed to determine and differentiate the composition of the secretomes of Phanerochaete chrysosporium CIRM-BRFM41, a peroxidase hypersecretory strain grown under ligninolytic conditions and on softwood chips under biopulping conditions. Extracellular proteins from both cultures were analyzed by bidimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry. A total of 37 spots were identified. The secretome in liquid synthetic medium comprised mainly peroxidases, while several wood-degrading enzymes and enzymes involved in fungal metabolism were detected in biopulping cultures on softwood. This prompted an analysis of the impact of secretome modulation in the presence of softwood chips. Biotreated wood was submitted to kraft cooking and chemical bleaching using chlorine dioxide. The fungal pre-treatment led to a significant increase in pulp yield and a better bleachability of the pulp. This bleachability improvement could be explained by the production of specific lignocellulose-degrading enzymes.
进行蛋白质组学分析，以确定和区分黄孢原毛平革菌CIRM-BRFM41的分泌蛋白组的组成。该菌株是一种过氧化物酶超分泌菌株，在木质素分解条件下以及生物制浆条件下的软木片上生长。通过二维凝胶电泳和基质辅助激光解吸/电离飞行时间串联质谱对两种培养物的细胞外蛋白质进行分析。共鉴定出37个斑点。液体合成培养基中的分泌蛋白组主要由过氧化物酶组成，而在软木生物制浆培养物中检测到几种木材降解酶和参与真菌代谢的酶。这促使对软木片存在下分泌蛋白组调节的影响进行分析。对生物处理过的木材进行硫酸盐蒸煮并用二氧化氯进行化学漂白。真菌预处理导致纸浆得率显著提高，且纸浆的漂白性能更好。这种漂白性能的改善可以通过特定木质纤维素降解酶的产生来解释。
