Verné E, Vitale-Brovarone C, Bui E, Bianchi C L, Boccaccini A R
Department of Materials Science and Chemical Engineering, Politecnico di Torino, C.so Duca Degli Abruzzi 24, 10129 Torino, Italy.
J Biomed Mater Res A. 2009 Sep 15;90(4):981-92. doi: 10.1002/jbm.a.32153.
Different cleaning and silanization methods have been applied to bioactive glasses with the aim of covalently bonding bone morphogenetic proteins (BMP-2) to the surface. Several glasses, with different bioactivity index, were cleaned with acidic, basic, or neutral aqueous media to investigate the role of pH in the formation of silanols on glass surfaces of different reactivity. The cleaned glasses were then functionalized using 3-aminopropyl-triethoxysilane (APTS). After the optimization of the silanization procedure, proteins of different complexity were immobilized on the functionalized glasses. To optimize the protein immobilization, a model protein (carnosine) was first used, and the procedure was then used to bind human BMP-2. The glass surfaces were characterized during each step of the treatment by water contact angles and X-ray photoelectron analyses. The APTS functionalization was then used to immobilize bone morphogenetic protein on the bioactive glasses. This result suggested that such a treatment could be successfully used as an efficient alternative to systemic administration of transforming growth factors for the development of local delivery vehicle implants.
为了使骨形态发生蛋白（BMP - 2）与生物活性玻璃表面共价结合，人们采用了不同的清洗和硅烷化方法。使用具有不同生物活性指数的几种玻璃，用酸性、碱性或中性水性介质进行清洗，以研究pH值在不同反应活性的玻璃表面硅醇形成过程中的作用。然后，用3 - 氨丙基 - 三乙氧基硅烷（APTS）对清洗后的玻璃进行功能化处理。在优化硅烷化程序后，将不同复杂性的蛋白质固定在功能化玻璃上。为了优化蛋白质固定，首先使用一种模型蛋白质（肌肽），然后将该程序用于结合人BMP - 2。在处理的每个步骤中，通过水接触角和X射线光电子分析对玻璃表面进行表征。然后使用APTS功能化将骨形态发生蛋白固定在生物活性玻璃上。该结果表明，这种处理方法可作为全身施用转化生长因子的有效替代方法，用于开发局部递送载体植入物。
