Magyari Klára, Baia Lucian, Vulpoi Adriana, Simon Simion, Popescu Octavian, Simon Viorica
Faculty of Physics and Institute of Interdisciplinary Research in Bio-Nano-Sciences, Babes-Bolyai University, 400084, Cluj-Napoca, Romania.
J Biomed Mater Res B Appl Biomater. 2015 Feb;103(2):261-72. doi: 10.1002/jbm.b.33203. Epub 2014 May 13.
The formation of a calcium phosphate layer on the surface of the SiO2 -CaO-P2 O5 glasses after immersion in simulated body fluid (SBF) generally demonstrates the bioactivity of these materials. Grafting of the surface by chemical bonding can minimize the structural changes in protein adsorbed on the surface. Therefore, in this study our interest was to evaluate the bioactivity and blood biocompatibility of the SiO2 -CaO-P2 O5 glasses after their surface modification by functionalization with aminopropyl-triethoxysilane and/or by fibrinogen. It is shown that the fibrinogen adsorbed on the glass surfaces induces a growing of the apatite-like layer. It is also evidenced that the protein content from SBF influences the growth of the apatite-like layer. Furthermore, the good blood compatibility of the materials after fibrinogen and bovine serum albumin adsorption is proved from the assessment of the β-sheet-β-turn ratio.
将SiO2 -CaO-P2 O5玻璃浸入模拟体液（SBF）后，其表面形成磷酸钙层通常表明这些材料具有生物活性。通过化学键合进行表面接枝可以使吸附在表面的蛋白质的结构变化最小化。因此，在本研究中，我们感兴趣的是评估经氨丙基三乙氧基硅烷功能化和/或纤维蛋白原处理后SiO2 -CaO-P2 O5玻璃的生物活性和血液生物相容性。结果表明，吸附在玻璃表面的纤维蛋白原会诱导类磷灰石层的生长。还证明了来自SBF的蛋白质含量会影响类磷灰石层的生长。此外，通过对β-折叠-β-转角比率的评估，证明了材料在吸附纤维蛋白原和牛血清白蛋白后具有良好的血液相容性。
