Wellehan James F X, Childress April L, Marschang Rachel E, Johnson April J, Lamirande Elaine W, Roberts John F, Vickers Mary L, Gaskin Jack M, Jacobson Elliott R
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA.
Vet Microbiol. 2009 Jan 1;133(1-2):34-42. doi: 10.1016/j.vetmic.2008.06.011. Epub 2008 Jun 21.
The orthoreoviruses are segmented RNA viruses that infect diverse vertebrate host species. While the most common human orthoreovirus, Mammalian Reovirus, is not typically associated with significant disease, the majority of Orthoreovirus species have been shown to cause significant and often fatal disease in reptiles, birds, and primates. There is significant potential for jumping species. A consensus nested-PCR method was designed for investigation of the RNA-dependent RNA polymerase gene of Orthoreovirus and Aquareovirus. This protocol was used to obtain sequencing template from reoviruses of three different vertebrate classes. Bayesian and maximum likelihood phylogenetic analysis found that all viruses analyzed clustered in the genus Orthoreovirus, that reptile reoviruses formed three distinct clusters, and that an African grey parrot reovirus clustered with Nelson Bay virus from bats. This PCR method may be useful for obtaining templates for initial sequencing of novel orthoreoviruses from diverse vertebrate hosts.
正呼肠孤病毒是分段RNA病毒，可感染多种脊椎动物宿主物种。虽然最常见的人类正呼肠孤病毒，即哺乳动物呼肠孤病毒，通常与严重疾病无关，但大多数正呼肠孤病毒物种已被证明可在爬行动物、鸟类和灵长类动物中引起严重且往往致命的疾病。存在显著的跨物种传播潜力。设计了一种共识巢式PCR方法，用于研究正呼肠孤病毒和水生呼肠孤病毒的RNA依赖性RNA聚合酶基因。该方案用于从三种不同脊椎动物类别的呼肠孤病毒中获取测序模板。贝叶斯和最大似然系统发育分析发现，所有分析的病毒都聚集在正呼肠孤病毒属中，爬行动物呼肠孤病毒形成了三个不同的簇，并且一只非洲灰鹦鹉呼肠孤病毒与来自蝙蝠的纳尔逊湾病毒聚集在一起。这种PCR方法可能有助于从不同脊椎动物宿主中获取新型正呼肠孤病毒初始测序的模板。
