Radu Elena, Fertig Tudor Emanuel, Dragu Laura Denisa, Pitică Ioana Mădălina, Surleac Marius, Neagu Ana Iulia, Pană Lavinia, Păiş Alexandra, Matei Lilia, Antone-Iordache Ionut-Lucian, Marta Daciana Silvia, Peteu Victor-Eduard, Niţă-Lazăr Mihai, Stoica Cătălina, Popescu Cornel, Sultana Camelia Mădălina, Botezatu Anca, Iancu Iulia Virginia, Chivu-Economescu Mihaela, Banică Leontina, Petre Ana Sorinica, Paraschiv Simona, Gherghiceanu Mihaela, Ruta Simona Maria, Kreuzinger Norbert, Diaconu Carmen Cristina, Bleotu Coralia
Department of Cellular and Molecular Pathology, Stefan S. Nicolau Institute of Virology, Bucharest, Romania.
Institute for Water Quality and Resource Management, Vienna University of Technology, Vienna, Austria.
J Cell Mol Med. 2025 Sep;29(17):e70805. doi: 10.1111/jcmm.70805.
Our study presents wastewater (WW) monitoring data, focusing on determining the infectivity of SARS-CoV-2 in the collected samples. Additionally, a panel of different viruses has been tested in the WW samples. The untreated WW monitoring campaign took place over 1 year in Bucharest, with approximately 300 samples being collected twice a week at the wastewater treatment plant (WWTP) and an infectious diseases hospital. Our data indicated that SARS-CoV-2 concentrations in WW preceded the increase in the number of clinical cases by nearly 2 weeks. Differences between locations were notable, with higher raw concentrations in WW samples from the hospital than those from the WWTP. However, after normalising to population equivalent, the hospital samples concentrations dropped significantly, suggesting that WW monitoring at the urban level provides a more complete and epidemiologically relevant picture than data obtained only from hospitals. Only a few isolates could demonstrate SARS-CoV-2 persistence during in vitro passages. Although the success rate was low, the technique remains crucial for validating the viability of viruses. Adenovirus, Bocavirus and Reovirus were the most abundant ones in both urban and hospital wastewater. WW monitoring remains the most effective approach for tracking the dissemination of various pathogens and supporting public health authorities.
我们的研究展示了废水监测数据，重点是确定收集样本中严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的传染性。此外，还对一组不同的病毒在废水样本中进行了检测。未经处理的废水监测活动在布加勒斯特进行了1年，每周两次在废水处理厂和一家传染病医院采集约300个样本。我们的数据表明，废水中SARS-CoV-2的浓度比临床病例数增加提前了近2周。不同地点之间的差异显著，医院废水样本中的原始浓度高于废水处理厂的样本。然而，在归一化为人口当量后，医院样本浓度显著下降，这表明城市层面的废水监测比仅从医院获得的数据提供了更完整且与流行病学相关的情况。只有少数分离株在体外传代过程中能显示出SARS-CoV-2的持久性。尽管成功率很低，但该技术对于验证病毒的活力仍然至关重要。腺病毒、博卡病毒和呼肠孤病毒在城市和医院废水中都是最丰富的。废水监测仍然是追踪各种病原体传播并为公共卫生当局提供支持的最有效方法。
