Bariszlovich M, Meusel D, Tülsner M
Institut für Lebensmittelchemie der Sektion Chemie, Humboldt-Universität zu Berlin.
Nahrung. 1991;35(1):61-9. doi: 10.1002/food.19910350117.
Types of lipase specificity are as follows: Positional specificity; fatty acid specificity; stereospecificity; substrate specificity (different rates of lipolysis of different glyceride classes. The acylglycerol used for determination of lipase specificity must be so structured, that specificities are not confused and unambiguous results are obtained. Different substrates and methods for detection of specificity are reviewed and advantages and disadvantages are discussed. Positional specificity can be determined with synthetic dialkylacylglycerols and 2,3-dioleoyl butanediol. Stereospecificity can be detected with enantiomeric dialkylacylglycerols or diacylalkylglycerols.
位置特异性；脂肪酸特异性；立体特异性；底物特异性（不同甘油酯类的脂解速率不同。用于测定脂肪酶特异性的酰基甘油必须具有这样的结构，即特异性不会混淆且能获得明确的结果。综述了检测特异性的不同底物和方法，并讨论了其优缺点。位置特异性可用合成二烷基酰基甘油和2,3 - 二油酰丁二醇来测定。立体特异性可用对映体二烷基酰基甘油或二酰基烷基甘油来检测。
