Shimosato Y, Kameya T, Nagai K, Hirohashi S, Koide T, Hayashi H, Nomura T
J Natl Cancer Inst. 1976 Jun;56(6):1251-60. doi: 10.1093/jnci/56.6.1251.
Ninety-one human tumors, including various common carcinomas, low-grade malignant tumors, and benign tumors, were transplanted into athymic nude mice. Tumor take was confirmed histologically for 22 neoplasms at the initial transplantation, and 14 serially transplantable tumors were established, including some hitherto unestablished or unreported, such as lung and hepatic cell carcinomas. Among the 91 tumors were 21, 14, and 13 carcinomas of the lung, stomach, and breast, respectively. Transplantability was highest in lung carcinomas (10/21), followed by gastric carcinomas (2/14) and breast carcinomas (1/13). Morphology of original tumors was retained well in most transplanted tumors, but desmoplastic or scirrhous tumors, such as gastric and breast carcinomas, tended to become medullary with a decrease in amount of tumor stroma. The ability to produce mucin in gastric carcinomas or melanin in malignant melanoma was maintained in serially transplantable tumors. In addition, ectopic production of adrenocorticotropin and beta melanocyte-stimulating hormone continued in a transplanted small cell carcinoma of the lung. Preliminary results were obtained on hormone dependency of the transplantable breast carcinoma and on alpha1-fetoprotein in the transplantable hepatic cell carcinoma.
91个人类肿瘤，包括各种常见的癌、低度恶性肿瘤和良性肿瘤，被移植到无胸腺裸鼠体内。在初次移植时，经组织学确认有22个肿瘤移植成功，建立了14个可连续传代移植的肿瘤模型，其中包括一些此前未建立或未报道过的肿瘤，如肺癌和肝细胞癌。在这91个肿瘤中，分别有21个、14个和13个肺癌、胃癌和乳腺癌。肺癌的移植成功率最高（10/21），其次是胃癌（2/14）和乳腺癌（1/13）。大多数移植肿瘤很好地保留了原发肿瘤的形态，但促结缔组织增生性或硬癌性肿瘤，如胃癌和乳腺癌，往往会变成髓样，肿瘤间质数量减少。在可连续传代移植的肿瘤中，胃癌产生黏液或恶性黑色素瘤产生黑色素的能力得以维持。此外，在移植的肺小细胞癌中，促肾上腺皮质激素和β-黑素细胞刺激素的异位分泌持续存在。获得了关于可移植乳腺癌的激素依赖性和可移植肝细胞癌中甲胎蛋白的初步结果。
