Dostal V, Kroath H, Binder M
Osterr Z Onkol. 1976 May;3(1-2):3-8.
Infection of CV-1 cell cultures with HSV-2 at MOI 5 respectively 1PFU/cell resulted in markedly different patterns of DNA-synthesis. Isolation and separation of cellular and viral DNA on CsCl equilibrium density gradients during the "late" phase after infection with 5PFU/cell revealed a rapid increase in synthesis of virus-specific DNA (which banded at density q25 = 1.729 g/ccm) while synthesis of host cell DNA (banding at 1.705 g/ccm) was constantly inhibited. 15 hours after infection and incubation with labelled medium, around infection and incubation with labelled medium, around 75% of total isolated DNA was virus-specific. On lowering the MOI to 1 PFU/cell, however, synthesis of host cell DNA continued and was even partially stimulated during the late phase whereas synthesis of virus-specific DNA advanced only slowly. 16 hours after infection, approximately 25% of the total assayed after infection, approximately 25% of the total assayed DNA was virus-specific and the amount of host cell DNA approached values of uninfected control cells. 24 hours after infection and incubation, virus specific DNA rose to approximately 45% of overall DNA assayed, and synthesis of host cell DNA was completely inhibited. However, infectivity rose constantly and reached 106.8 TCD 50/ml at 22 hours p.i. These findings were independent of passage number of CV-cell culture, and there were no alterations in karyotype and morphological behaviour of the cells.
以5个感染复数（MOI）即每细胞1个空斑形成单位（PFU）的HSV - 2感染CV - 1细胞培养物，导致DNA合成模式明显不同。在用每细胞5个PFU感染后的“晚期”，通过氯化铯平衡密度梯度对细胞和病毒DNA进行分离，结果显示病毒特异性DNA（密度为q25 = 1.729 g/ccm处形成条带）的合成迅速增加，而宿主细胞DNA（密度为1.705 g/ccm处形成条带）的合成持续受到抑制。在感染并与标记培养基孵育15小时后，即感染并与标记培养基孵育前后，分离得到的总DNA中约75%是病毒特异性的。然而，当将MOI降至每细胞1个PFU时，宿主细胞DNA的合成在晚期仍继续，甚至部分受到刺激，而病毒特异性DNA的合成进展缓慢。感染16小时后，感染后检测的总DNA中约25%是病毒特异性的，宿主细胞DNA的量接近未感染对照细胞的值。感染并孵育24小时后，病毒特异性DNA升至检测的总DNA的约45%，宿主细胞DNA的合成被完全抑制。然而，感染性持续上升，在感染后22小时达到106.8半数组织培养感染剂量（TCD50）/毫升。这些发现与CV - 1细胞培养物的传代次数无关，并且细胞的核型和形态行为没有改变。
