从水生拉恩氏菌33071T脂多糖中分离并鉴定两种不同的多糖。
Isolation and structure elucidation of two different polysaccharides from the lipopolysaccharide of Rahnella aquatilis 33071T.
作者信息
Zdorovenko Evelina L, Varbanets Ludmila D, Zatonsky George V, Zdorovenko Galina M, Shashkov Alexander S, Knirel Yuriy A
机构信息
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospeckt 47, 119991 Moscow, Russia.
出版信息
Carbohydr Res. 2009 Jul 6;344(10):1259-62. doi: 10.1016/j.carres.2009.04.013. Epub 2009 Apr 17.
Two different polysaccharides were obtained by mild acid degradation of the lipopolysaccharide of Rahnella aquatilis 33071(T). These were studied by sugar and methylation analyses along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structures were established for the polysaccharides: -->4)-alpha-D-Rhap-(1-->3)-alpha-D-Rhap-(1-->3)beta-D-Galp-(1-->3)-alpha-D-Manp-(1-->2)-alpha-D-Manp-(1--> beta-D-Glcp-(1-->3)-alpha-D-galp-(1-->4)-alpha-D-GlcpA-(1-->2). The former structure is new, whereas the latter has been reported earlier as the structure of the O-specific polysaccharide of R. aquatilis 95 U003 (Zdorovenko, E. L.; Varbanets, L. D.; Zatonsky, G. V.; Kachala, V. V.; Zdorovenko, G. M.; Shashkov, A. S.; Knirel, Y. A. Carbohydr. Res.2008, 343, 2494-2497).
通过温和酸降解水生拉恩菌33071(T)的脂多糖获得了两种不同的多糖。通过糖分析、甲基化分析以及一维和二维（1）H和（13）C NMR光谱对其进行了研究。确定了这些多糖的以下结构：→4)-α-D-鼠李糖-(1→3)-α-D-鼠李糖-(1→3)β-D-半乳糖-(1→3)-α-D-甘露糖-(1→2)-α-D-甘露糖-(1→β-D-葡萄糖-(1→3)-α-D-半乳糖-(1→4)-α-D-葡萄糖醛酸-(1→2)。前一种结构是新的，而后者早些时候已作为水生拉恩菌95 U003的O-特异性多糖的结构被报道过（兹多罗文科，E.L.；瓦尔巴涅茨，L.D.；扎通斯基，G.V.；卡查拉，V.V.；兹多罗文科，G.M.；沙什科夫，A.S.；克尼雷尔，Y.A.《碳水化合物研究》2008年，343卷，2494 - 2497页）。
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