Lee Jae-Chan, Li Wen-Jun, Xu Li-Hua, Jiang Cheng-Lin, Kim Chang-Jin
Functional Metabolomics Research Center, KRIBB, Daejeon, Republic of Korea.
Int J Syst Evol Microbiol. 2008 Aug;58(Pt 8):1838-43. doi: 10.1099/ijs.0.65545-0.
A Gram-positive, moderately halophilic bacterium, designated strain BH113T, was isolated from a salt lake located in Xinjiang Province, China. Cells of the strain were aerobic, spore-forming, motile rods with flagella. The organism grew optimally at 37 degrees C and pH 8.0 in the presence of 10 % (w/v) NaCl. A phylogenetic analysis based on comparisons of 16S rRNA gene sequences showed that the isolate formed a clade with the type strains of the genus Lentibacillus. The levels of 16S rRNA gene sequence similarity between strain BH113T and Lentibacillus lacisalsi KCTC 3915T, Lentibacillus juripiscarius JCM 12147T and Lentibacillus kapialis JCM 12580T were 96.7, 96.3 and 96.1 %, respectively. The G+C content of the genomic DNA was 46.2 mol% and the major isoprenoid quinone was MK-7. The peptidoglycan type was A1gamma (meso-diaminopimelic acid) and the major polar lipids were phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0. Therefore, on the basis of physiological, chemotaxonomic and molecular properties, strain BH113T represents a novel species within the genus Lentibacillus, for which the name Lentibacillus salis sp. nov. is proposed. The type strain is BH113T (=KCTC 3936T =DSM 16817T).
从中国新疆的一个盐湖中分离出一株革兰氏阳性中度嗜盐细菌，命名为BH113T菌株。该菌株的细胞为需氧、产芽孢、具鞭毛的运动杆状。该菌在含有10%（w/v）NaCl的条件下，最适生长温度为37℃，最适pH为8.0。基于16S rRNA基因序列比较的系统发育分析表明，该分离株与Lentibacillus属的模式菌株形成一个进化分支。BH113T菌株与Lentibacillus lacisalsi KCTC 3915T、Lentibacillus juripiscarius JCM 12147T和Lentibacillus kapialis JCM 12580T的16S rRNA基因序列相似性水平分别为96.7%、96.3%和96.1%。基因组DNA的G+C含量为46.2 mol%，主要类异戊二烯醌为MK-7。肽聚糖类型为A1γ（内消旋二氨基庚二酸），主要极性脂为磷脂酰甘油和双磷脂酰甘油。主要脂肪酸为anteiso-C15:0、iso-C16:0和anteiso-C17:0。因此，基于生理、化学分类和分子特性，BH113T菌株代表Lentibacillus属内的一个新物种，为此提出Lentibacillus salis sp. nov.的名称。模式菌株为BH113T（=KCTC 3936T =DSM 16817T）。
