Bongiorno-Borbone Lucilla, De Cola Antonella, Vernole Patrizia, Finos Livio, Barcaroli Daniela, Knight Richard A, Melino Gerry, De Laurenzi Vincenzo
Department of Experimental Medicine, University of Rome Tor Vergata, Roma, Italy.
Cell Cycle. 2008 Aug;7(15):2357-67. doi: 10.4161/cc.6344. Epub 2008 May 21.
Cajal Bodies are one of many specialised organelles contained in the eukaryotic cell nucleus, and are involved in a number of functions, including regulation of replication-dependent histone gene transcription. In normal diploid cells their number varies between 0 and 4 depending on the cell cycle phase, although in cancer cell lines their number is extremely variable and it has been suggested that it correlates with cell ploidy. Here we show that in mammalian cells, as in Drosophila, two distinct though functionally related bodies exist: a histone gene locus body and a Cajal Body. The first one can be detected using FLASH or NPAT as markers while the second is labelled using antibodies against Coilin. Only the number of FLASH/NPAT histone gene locus bodies correlates with ploidy and only these organelles appear to be regulated during the cell cycle. Finally, we show that the two organelles completely co-localize during the S phase of the cell cycle.
卡哈尔体是真核细胞核中包含的众多特殊细胞器之一，参与多种功能，包括对依赖复制的组蛋白基因转录的调控。在正常二倍体细胞中，其数量根据细胞周期阶段在0到4之间变化，尽管在癌细胞系中其数量变化极大，并且有人提出它与细胞倍性相关。在这里我们表明，在哺乳动物细胞中，如同在果蝇中一样，存在两种不同但功能相关的小体：组蛋白基因位点小体和卡哈尔体。第一种可以使用FLASH或NPAT作为标记物检测到，而第二种则使用抗卷曲螺旋蛋白的抗体进行标记。只有FLASH/NPAT组蛋白基因位点小体的数量与倍性相关，并且只有这些细胞器在细胞周期中似乎受到调控。最后，我们表明这两种细胞器在细胞周期的S期完全共定位。
