Yuuki Kenji, Tsukasaki Hiroaki, Kawawa Tadaharu, Shiba Akihiko, Shiba Kiyoko
Department of Prosthodontics, Showa University School of Dentistry.
Nihon Hotetsu Shika Gakkai Zasshi. 2008 Jul;52(3):340-9. doi: 10.2186/jjps.52.340.
Clinical findings were compared with glucose, protein, albumin, bilirubin, creatinine, pH, occult blood, ketone body, nitrite, and white blood cells contained in whole saliva to investigate the components that most markedly reflect the periodontal condition.
The subjects were staff of the Prosthodontics Department, Showa University, and patients who visited for dental treatments (57 subjects in total). At the first time, saliva samples were gargled with 1.5 ml of distilled water for 15 seconds and collected by spitting out into a paper cup. At the second time, saliva samples were collected by the same method. At the third time, saliva samples after chewing paraffin gum for 60 seconds were collected by spitting out into a paper cup. Thus whole saliva collecting that was divided on three times. After sampling, 8 mul of the saliva sample was dripped in reagent sticks for the 10 items of urinary test paper and the reflectance was measured using a specific reflectometer. In the periodontal tissue evaluation, the degree of alveolar bone resorption, probing value, and tooth mobility and the presence or absence of lesions in the root furcation were examined and classified into 4 ranks. The mean values in each periodontal disease rank and correlation between the periodontal disease ranks and the components were statistically analyzed.
Bilirubin and ketone body were not measurable. The components density of the 8 items was increased as the periodontal disease rank increased. Regarding the correlation between the periodontal disease ranks and the components, high correlations were noted for protein, albumin, creatinine, pH, and white blood cells.
The simultaneous measurement method of 8 salivary components using test paper may be very useful for the diagnosis of periodontal disease of abutment teeth.
将临床检查结果与全唾液中的葡萄糖、蛋白质、白蛋白、胆红素、肌酐、pH值、潜血、酮体、亚硝酸盐和白细胞进行比较，以研究最能显著反映牙周状况的成分。
研究对象为昭和大学口腔修复科工作人员及前来接受牙科治疗的患者（共57名受试者）。第一次，用1.5毫升蒸馏水漱口15秒，然后吐入纸杯收集唾液样本。第二次，采用相同方法收集唾液样本。第三次，咀嚼石蜡口香糖60秒后，将唾液样本吐入纸杯收集。如此分三次收集全唾液。采样后，将8微升唾液样本滴在尿试纸的10项试剂条上，并用特定的反射仪测量反射率。在牙周组织评估中，检查牙槽骨吸收程度、探诊值、牙齿松动度以及根分叉处有无病变，并分为4个等级。对各牙周疾病等级的平均值以及牙周疾病等级与各成分之间的相关性进行统计学分析。
胆红素和酮体无法测量。随着牙周疾病等级的升高，8项成分的密度增加。关于牙周疾病等级与各成分之间的相关性，蛋白质、白蛋白、肌酐、pH值和白细胞的相关性较高。
使用试纸同时测量8种唾液成分的方法可能对基牙牙周疾病的诊断非常有用。
